Epigenetic alterations such as DNA methylation have been associated with the etiology of inflammation-related diseases. The present study evaluated the association between the methylation levels of LPL , ADRB3 and MTHFR genes and the stage of inflammation in individuals with isolated or associated morbidities, as well as in individuals without morbidities. This is a cross-sectional population-based study, in which 261 adults, between 20 and 59 years, individuals of both sex were selected. Inflammatory parameters were evaluated in blood, and the evaluation of methylation levels in the promoter of LPL , ADRB3 and MTHFR genes was performed in peripheral blood leukocytes. For statistical treatment, normality analysis was performed using the Lilliefors test, multiple linear regression, in addition to odds ratio. For all tests, the significance level adopted was 5%. In individuals with isolated morbidities, a positive association was observed between CRP values (2.49mg/L±3.7) and LPL gene methylation levels (35%±18) (p=.003) and with associated morbidities, females had higher levels of LPL gene methylation (40% ± 20) (p = 0.041) and for MTHFR gene methylation levels (35% ± 18) a positive association was found with MDA values (3.02µmol/L± 0.8) (p=.032). In addition, the use of medications did not influence the level of methylation for any of the three genes analyzed. Among individuals with isolated or associated morbidities, there was an association between the methylation levels of the LPL gene with the CRP values and females, and furthermore with the MTHFR gene and MDA. This study could help to understand the etiology and treatment of different morbidities, enabling the discovery of new combats resources.