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Research article
Hem Chandra Jha
Indian Institute of Technology Indore
Corresponding Author
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Background Helicobacter pylori (H. pylori) is well-known for its role in chronic gastritis and gastric cancer. Eradication of these carcinogenic bacteria from the gut is one of the major challenges for the clinicians. The complexity of treatment is mainly owing to, antibiotic resistance and relapse due to extra gastric reservoir in the oral cavity. Our study focuses on the isolation of H. pylori from distinct habitats (gastric biopsy and gastric juice) of the gut microenvironment and its subsequent characterization. Further, we have also evaluated the effect of various plaque control agents on isolated strains of H. pylori.Results The strains isolated from two different habitats of the same subject shows a striking difference in their growth pattern. Promisingly, some included chemicals are effective in growth inhibition after recommended 30 sec treatment. The subsequent evaluation shows that chemical B (out of chemical A, B, C, D and E) is the most effective and downregulate the expression of CagA in the infected AGS cells.Conclusion For the fast time, in our study the pylori isolated from different habitat of the same subject shows differential growth pattern. Nowadays, antibiotic resistance and relapse of H. pylori make it difficult to eradicate the bacteria from gastric as well as oral reservoir. Thus, this study may help to reduce the bacteria in their oral reservoir and also control the H. pylori migration from oral to gastric compartment.
Keywords
Gastric cancer, Plaque control agent, CagA, Apoptotic pathway, Growth curve
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Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Microbiology.
Community comments: 2
Editorial decision: Accept
On 13 Feb, 2020
Editor assigned
On 12 Feb, 2020
Submission checks complete
On 11 Feb, 2020
Editor invited
On 11 Feb, 2020
No comments provided
Editorial decision: Minor revision
On 10 Feb, 2020
Editor assigned
On 27 Jan, 2020
Submission checks complete
On 26 Jan, 2020
Editor invited
On 26 Jan, 2020
No comments provided
Editorial decision: Major revision
On 17 Jan, 2020
Editor assigned
On 03 Jan, 2020
Submission checks complete
On 02 Jan, 2020
Editor invited
On 02 Jan, 2020
Version 1
Posted 06 Nov, 2019
No comments provided
Review #2 received
Received 12 Dec, 2019
Editorial decision: Major revision
On 12 Dec, 2019
Review #1 received
Received 05 Dec, 2019
Reviewer #2 agreed
On 29 Nov, 2019
Reviewer #1 agreed
On 13 Nov, 2019
Reviewers invited
Invitations sent on 08 Nov, 2019
Submission checks complete
On 03 Nov, 2019
Editor assigned
On 29 Oct, 2019
Editor invited
On 28 Oct, 2019
First submitted
On 26 Oct, 2019
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A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
See the published version of this preprint at BMC Microbiology.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Hem Chandra Jha
Indian Institute of Technology Indore
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Microbiology.
Community comments: 2
Editorial decision: Accept
On 13 Feb, 2020
Editor assigned
On 12 Feb, 2020
Submission checks complete
On 11 Feb, 2020
Editor invited
On 11 Feb, 2020
No comments provided
Editorial decision: Minor revision
On 10 Feb, 2020
Editor assigned
On 27 Jan, 2020
Submission checks complete
On 26 Jan, 2020
Editor invited
On 26 Jan, 2020
No comments provided
Editorial decision: Major revision
On 17 Jan, 2020
Editor assigned
On 03 Jan, 2020
Submission checks complete
On 02 Jan, 2020
Editor invited
On 02 Jan, 2020
Version 1
Posted 06 Nov, 2019
No comments provided
Review #2 received
Received 12 Dec, 2019
Editorial decision: Major revision
On 12 Dec, 2019
Review #1 received
Received 05 Dec, 2019
Reviewer #2 agreed
On 29 Nov, 2019
Reviewer #1 agreed
On 13 Nov, 2019
Reviewers invited
Invitations sent on 08 Nov, 2019
Submission checks complete
On 03 Nov, 2019
Editor assigned
On 29 Oct, 2019
Editor invited
On 28 Oct, 2019
First submitted
On 26 Oct, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Microbiology.
Background Helicobacter pylori (H. pylori) is well-known for its role in chronic gastritis and gastric cancer. Eradication of these carcinogenic bacteria from the gut is one of the major challenges for the clinicians. The complexity of treatment is mainly owing to, antibiotic resistance and relapse due to extra gastric reservoir in the oral cavity. Our study focuses on the isolation of H. pylori from distinct habitats (gastric biopsy and gastric juice) of the gut microenvironment and its subsequent characterization. Further, we have also evaluated the effect of various plaque control agents on isolated strains of H. pylori.Results The strains isolated from two different habitats of the same subject shows a striking difference in their growth pattern. Promisingly, some included chemicals are effective in growth inhibition after recommended 30 sec treatment. The subsequent evaluation shows that chemical B (out of chemical A, B, C, D and E) is the most effective and downregulate the expression of CagA in the infected AGS cells.Conclusion For the fast time, in our study the pylori isolated from different habitat of the same subject shows differential growth pattern. Nowadays, antibiotic resistance and relapse of H. pylori make it difficult to eradicate the bacteria from gastric as well as oral reservoir. Thus, this study may help to reduce the bacteria in their oral reservoir and also control the H. pylori migration from oral to gastric compartment.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
This is a list of supplementary files associated with this preprint. Click to download.
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