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Research article
Ai-Qun JIA
Nanjing University of Science and Technology
Corresponding Author
ORCiD: https://orcid.org/0000-0002-8089-6200
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background Xanthomonas oryzae pv. oryzae ( Xoo ) can cause destructive bacterial blight in rice. As an antibacterial, resveratrol may inhibit Xoo growth. This study focused on the potential structural-activity relationship of resveratrol and its derivatives against Xoo growth, and 1 H-NMR-based metabolomic analysis was applied to investigate the global metabolite changes in Xoo after resveratrol treatment.
Results Resveratrol showed the strongest inhibitory effects on Xoo growth compared with its derivatives, which lacked double bonds (compounds 4 – 6 ) or hydroxyls were substituted with methoxyls (compounds 7 – 9 ). The IC 50 of resveratrol against Xoo growth was 11.67 ± 0.58 μg/mL. Results indicated that the double bond of resveratrol contributed to its inhibitory effects on Xoo growth, and hydroxyls were vital for this inhibition. Interestingly, resveratrol also significantly inhibited Xoo flagellum growth. Based on 1 H-NMR global metabolic analysis, a total of 30 Xoo metabolites were identified, the changes in the metabolic profile indicated that resveratrol could cause oxidative stress as well as disturb energy, purine, amino acid, and NAD + metabolism in Xoo , resulting in the observed inhibitory effects on growth.
Conclusions This study showed that the double bond of resveratrol contributed to its inhibitory effects on Xoo growth, and hydroxyls were also the important active groups. Resveratrol could cause oxidative stress of Xoo cells, and disturb the metabolism of energy, purine, amino acid and NAD +, thus inhibit Xoo growth.
Keywords
Xanthomonas oryzae pv. Oryzae, Resveratrol, Flagella, Structure-activity relationship, Global metabolic analysis
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Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Microbiology.
Version 2
Posted 18 Mar, 2020
No community comments so far
Editorial decision: Accept
On 24 Apr, 2020
Review #2 received
Received 02 Apr, 2020
Reviewer #2 agreed
On 01 Apr, 2020
Reviewer #1 agreed
On 31 Mar, 2020
Review #1 received
Received 31 Mar, 2020
Reviewers invited
Invitations sent on 27 Mar, 2020
Editor assigned
On 19 Mar, 2020
Editor invited
On 18 Mar, 2020
Submission checks complete
On 13 Mar, 2020
No comments provided
Editorial decision: Major revision
On 14 Feb, 2020
Review #3 received
Received 11 Feb, 2020
Reviewer #3 agreed
On 09 Feb, 2020
Review #2 received
Received 20 Jan, 2020
Reviewer #2 agreed
On 05 Jan, 2020
Review #1 received
Received 17 Dec, 2019
Reviewer #1 agreed
On 13 Dec, 2019
Reviewers invited
Invitations sent on 12 Dec, 2019
Submission checks complete
On 03 Nov, 2019
Editor assigned
On 31 Oct, 2019
Editor invited
On 30 Oct, 2019
First submitted
On 29 Oct, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
General Microbiology
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A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
See the published version of this preprint at BMC Microbiology.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Ai-Qun JIA
Nanjing University of Science and Technology
Corresponding Author
ORCiD: https://orcid.org/0000-0002-8089-6200
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Microbiology.
Version 2
Posted 18 Mar, 2020
No community comments so far
Editorial decision: Accept
On 24 Apr, 2020
Review #2 received
Received 02 Apr, 2020
Reviewer #2 agreed
On 01 Apr, 2020
Reviewer #1 agreed
On 31 Mar, 2020
Review #1 received
Received 31 Mar, 2020
Reviewers invited
Invitations sent on 27 Mar, 2020
Editor assigned
On 19 Mar, 2020
Editor invited
On 18 Mar, 2020
Submission checks complete
On 13 Mar, 2020
No comments provided
Editorial decision: Major revision
On 14 Feb, 2020
Review #3 received
Received 11 Feb, 2020
Reviewer #3 agreed
On 09 Feb, 2020
Review #2 received
Received 20 Jan, 2020
Reviewer #2 agreed
On 05 Jan, 2020
Review #1 received
Received 17 Dec, 2019
Reviewer #1 agreed
On 13 Dec, 2019
Reviewers invited
Invitations sent on 12 Dec, 2019
Submission checks complete
On 03 Nov, 2019
Editor assigned
On 31 Oct, 2019
Editor invited
On 30 Oct, 2019
First submitted
On 29 Oct, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
General Microbiology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Microbiology.
Background Xanthomonas oryzae pv. oryzae ( Xoo ) can cause destructive bacterial blight in rice. As an antibacterial, resveratrol may inhibit Xoo growth. This study focused on the potential structural-activity relationship of resveratrol and its derivatives against Xoo growth, and 1 H-NMR-based metabolomic analysis was applied to investigate the global metabolite changes in Xoo after resveratrol treatment.
Results Resveratrol showed the strongest inhibitory effects on Xoo growth compared with its derivatives, which lacked double bonds (compounds 4 – 6 ) or hydroxyls were substituted with methoxyls (compounds 7 – 9 ). The IC 50 of resveratrol against Xoo growth was 11.67 ± 0.58 μg/mL. Results indicated that the double bond of resveratrol contributed to its inhibitory effects on Xoo growth, and hydroxyls were vital for this inhibition. Interestingly, resveratrol also significantly inhibited Xoo flagellum growth. Based on 1 H-NMR global metabolic analysis, a total of 30 Xoo metabolites were identified, the changes in the metabolic profile indicated that resveratrol could cause oxidative stress as well as disturb energy, purine, amino acid, and NAD + metabolism in Xoo , resulting in the observed inhibitory effects on growth.
Conclusions This study showed that the double bond of resveratrol contributed to its inhibitory effects on Xoo growth, and hydroxyls were also the important active groups. Resveratrol could cause oxidative stress of Xoo cells, and disturb the metabolism of energy, purine, amino acid and NAD +, thus inhibit Xoo growth.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
This is a list of supplementary files associated with this preprint. Click to download.
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