Ethics approval and consent to participate
The regulatory authorities in Mozambique, United States of America and Belgium, the Mozambican National Ethics Committee (Comité Nacional de Bioética em Saúde) (282/CNBS/13), the Walter Reed Army Institute of Research (WRAIR) Institutional Review Board (WRAIR #2250), and the Medical Ethics Committee of the Institute of Tropical Medicine, Belgium (1065/15) approved this study. All participants consented to participate in this study.
Consent for publication
Availability of data and materials
The datasets used and analyzed during the current study are available from the corresponding author on reasonable request.
The authors have declared that no competing interests exist.
This work was supported by a cooperative agreement (W81XWH-07-2-0067) between the Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc., and the U.S. Department of Defense (DOD). This research was funded, in part, by the U.S. National Institute of Allergy and Infectious Diseases. Some funds were also received from the Flanders Department of Foreign Affairs of the Flemish Government (BICMINS). The funding sources had no involvement in: study design, data collection, analysis, interpretation of data, writing the manuscript nor in decision to submission of the article for publication. Material has been reviewed by the Walter Reed Army Institute of Research. There is no objection to its presentation and/or publication. The opinions or assertions contained herein are the private views of the author, and are not to be construed as official, or as reflecting true views of the Department of the Army or the Department of Defense. The investigators have adhered to the policies for protection of human subjects as prescribed in AR 70–25.
RMC, CM, MR, VM and VC performed the experiments. RMC, ME, IM, NB, CSP and MI participated in study coordination. CSP provided supervision, and CSP and MI provided project administration. RMC performed the data analysis, interpretation and drafted the manuscript. RMC, IJ and LK designed the study, conceived the experiments, and wrote the manuscript. All authors reviewed the manuscript, provided feedback, and approved of the manuscript in its final form.
We are thankful to all participants who made this work possible. Special thanks goes to Mark de Souza for his robust comments and reviews of this manuscript and the institutions that supported this work including the Instituto Nacional de Saúde (INS), Polana Caniço Health Research and Training Center (CISPOC), Henry M. Jackson Foundation for the Advancement of Military Medicine (HJF), HJF Medical Research International (HJFMRI), US Military HIV Research Program (MHRP), and the Walter Reed Army Institute of Research (WRAIR). The authors are also thankful to the study participants who made this work possible. The RV363 Study Group includes: Julie Ake, Khelvon De Araujo, Nilesh Bhatt, Igor Capitine, Raquel Matavele Chissumba, Alberto Machaze, Eduardo Namalamgo, Celso Castiano, Emelva Manhiça, Mirna Mutombene, Ducília Matimbe, Onélia Guiliche, Vania Mapossa, Vania Monteiro, Nilzio Cavele, Trevor Crowell, Leigh Anne Eller, Zebiba Hassen, Michelle Imbach, Luis Inhambizo, Ilesh V. Jani, Qun Li, Ivalda Macicame, Ferrao Mandlate, Carmélia Massingue, Mark Milazzo, Vanessa Monteiro, Chiaka Nwoga, Christina S. Polyak, Patrícia Ramgi, Merlin L. Robb, Steve Schech, Gail Smith, Edith M. Swann, Edna Viegas, and Adam Yates.