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Bone Marrow Mesenchymal Stem Cells Derived miRNA-130b Enhances Epithelial Sodium Channel by Targeting PTEN
Hongguang Nie
China Medical University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-9973-6354
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at Respiratory Research.
Aims: Acute lung injury (ALI) is a clinical syndrome with high morbidity and mortality, and severe pulmonary edema is one of the characteristics of ALI. Epithelial sodium channel (ENaC) located on the apical side of alveolar type 2 epithelial (AT2) cells is the primary rate limiting segments in alveolar fluid clearance. Many preclinical studies have revealed that mesenchymal stem cells (MSCs) based therapy has great therapeutic potential for ALI, while the role of ENaC in this process is rarely known.
Methods: We studied the effects of bone marrow-derived MSCs (BMSCs) on the protein/mRNA expression and activity of ENaC in primary mouse AT2 and H441 cells by co-culture with them, respectively. Moreover, the changes of miRNA-130b in AT2 cells were detected by qRT-PCR, and we studied the involvement of phosphatase and tensin homolog deleted on chromosome ten (PTEN) in miRNA-130b regulated ENaC.
Results: Our results demonstrated that BMSCs could increase the expression of miRNA-130b, which showed adverse effects on the protein expression of α/γ-ENaC and PTEN in AT2 cells. SiRNA-mediated downregulation of PTEN could increase the protein expression of α/γ-ENaC in AT2 cells, supporting PTEN as a negative regulator of ENaC.
Conclusion: In summary, miRNA-130b in BMSCs can enhance ENaC at least partially by targeting PTEN, which may provide a promising new direction for therapeutic strategy in ALI.
Keywords
epithelial sodium channels, bone marrow mesenchymal stem cells, miRNA-130b, acute lung injury
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CURRENT STATUS: Published
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Posted 21 Sep, 2020
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On 11 Dec, 2020
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Review #1 received
Received 05 Oct, 2020
Editorial decision: Major revision
On 05 Oct, 2020
Review #2 received
Received 27 Sep, 2020
Review #3 received
Received 27 Sep, 2020
Reviewer #4 agreed
On 24 Sep, 2020
Reviewer #3 agreed
On 23 Sep, 2020
Reviewers invited
Invitations sent on 22 Sep, 2020
Reviewer #1 agreed
On 22 Sep, 2020
Reviewer #2 agreed
On 22 Sep, 2020
Editor assigned
On 16 Sep, 2020
First submitted
On 15 Sep, 2020
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On 15 Sep, 2020
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This preprint is under consideration at Respiratory Research. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Bone Marrow Mesenchymal Stem Cells Derived miRNA-130b Enhances Epithelial Sodium Channel by Targeting PTEN
Hongguang Nie
China Medical University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-9973-6354
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Version 1
Posted 21 Sep, 2020
Published
On 11 Dec, 2020
No community comments so far
Review #1 received
Received 05 Oct, 2020
Editorial decision: Major revision
On 05 Oct, 2020
Review #2 received
Received 27 Sep, 2020
Review #3 received
Received 27 Sep, 2020
Reviewer #4 agreed
On 24 Sep, 2020
Reviewer #3 agreed
On 23 Sep, 2020
Reviewers invited
Invitations sent on 22 Sep, 2020
Reviewer #1 agreed
On 22 Sep, 2020
Reviewer #2 agreed
On 22 Sep, 2020
Editor assigned
On 16 Sep, 2020
First submitted
On 15 Sep, 2020
Submission checks complete
On 15 Sep, 2020
Editor invited
On 15 Sep, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at Respiratory Research.
Aims: Acute lung injury (ALI) is a clinical syndrome with high morbidity and mortality, and severe pulmonary edema is one of the characteristics of ALI. Epithelial sodium channel (ENaC) located on the apical side of alveolar type 2 epithelial (AT2) cells is the primary rate limiting segments in alveolar fluid clearance. Many preclinical studies have revealed that mesenchymal stem cells (MSCs) based therapy has great therapeutic potential for ALI, while the role of ENaC in this process is rarely known.
Methods: We studied the effects of bone marrow-derived MSCs (BMSCs) on the protein/mRNA expression and activity of ENaC in primary mouse AT2 and H441 cells by co-culture with them, respectively. Moreover, the changes of miRNA-130b in AT2 cells were detected by qRT-PCR, and we studied the involvement of phosphatase and tensin homolog deleted on chromosome ten (PTEN) in miRNA-130b regulated ENaC.
Results: Our results demonstrated that BMSCs could increase the expression of miRNA-130b, which showed adverse effects on the protein expression of α/γ-ENaC and PTEN in AT2 cells. SiRNA-mediated downregulation of PTEN could increase the protein expression of α/γ-ENaC in AT2 cells, supporting PTEN as a negative regulator of ENaC.
Conclusion: In summary, miRNA-130b in BMSCs can enhance ENaC at least partially by targeting PTEN, which may provide a promising new direction for therapeutic strategy in ALI.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Due to technical limitations, full-text HTML conversion of this manuscript could not be completed. However, the manuscript can be downloaded and accessed as a PDF.