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Research Article
Molecular Epidemiological Investigation and Analysis of Toxoplasma Gondii Infection in Livestock, Poultry, and Pets in Henan Province, Central China
Shiguo Liu
Xinxiang Medical University
Corresponding Author
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Background:Toxoplasma gondii (T.gondii ) is a protozoan parasite spread around the world and is concerned as one of the most important foodborne zoonotic parasites in the global world. This study aimed to determine the prevalence of T.gondii in foodborne animals in Henan Province, China.
Methods:During 2012 to 2015, a total of 1879 samples of foodborne animal tissues or peripheral blood were collected from 12 slaughterhouses, eight pastures and 434 farmers in different areas of Henan province using systematic random sampling method. The genomic DNA was extracted by the phenol-chloroform method. Moreover, then a One-tube nested polymerase chain reaction (PCR) assay was performed to amplify the 529bp repetitive sequence of T.gondii in these samples.T.gondii infections in diverse animals is different in the study.
Results:Of the 1879 samples,cat had the highest T.gondii infection(51.9%,28/54),followed by goose(24.3%,44/181), chicken(19.7%,91/462), cattle(12.6%,23/182), sheep(11.5%,22/191), duck(11.3%,24/212), dog(10.5%,13/124), pigeon(5.4%,16/297) and pig(4.6%,8/176). The total infection of T. gondii in these animals was 269/1879, and the positive rate was 14.3%.
Conclusions:The results revealed that all the livestock, poultry, and pets in close contact with humans had toxoplasma infection, which suggest that the consumption of meat products in Henan may pose a potential threat to human health. Therefore, to strengthen the prevention and control of T.gondii infection in animals is an important measure to develop animal husbandry, support food safety, and protect human health.
Keywords
Toxoplasma gondii, livestock, poultry, nested PCR, Henan, China
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This is a preprint. It has not completed peer review.
Research Article
Molecular Epidemiological Investigation and Analysis of Toxoplasma Gondii Infection in Livestock, Poultry, and Pets in Henan Province, Central China
Shiguo Liu
Xinxiang Medical University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 28 Sep, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background:Toxoplasma gondii (T.gondii ) is a protozoan parasite spread around the world and is concerned as one of the most important foodborne zoonotic parasites in the global world. This study aimed to determine the prevalence of T.gondii in foodborne animals in Henan Province, China.
Methods:During 2012 to 2015, a total of 1879 samples of foodborne animal tissues or peripheral blood were collected from 12 slaughterhouses, eight pastures and 434 farmers in different areas of Henan province using systematic random sampling method. The genomic DNA was extracted by the phenol-chloroform method. Moreover, then a One-tube nested polymerase chain reaction (PCR) assay was performed to amplify the 529bp repetitive sequence of T.gondii in these samples.T.gondii infections in diverse animals is different in the study.
Results:Of the 1879 samples,cat had the highest T.gondii infection(51.9%,28/54),followed by goose(24.3%,44/181), chicken(19.7%,91/462), cattle(12.6%,23/182), sheep(11.5%,22/191), duck(11.3%,24/212), dog(10.5%,13/124), pigeon(5.4%,16/297) and pig(4.6%,8/176). The total infection of T. gondii in these animals was 269/1879, and the positive rate was 14.3%.
Conclusions:The results revealed that all the livestock, poultry, and pets in close contact with humans had toxoplasma infection, which suggest that the consumption of meat products in Henan may pose a potential threat to human health. Therefore, to strengthen the prevention and control of T.gondii infection in animals is an important measure to develop animal husbandry, support food safety, and protect human health.
Figure 1
Figure 2
Figure 3