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Heterogeneity of Moraxella isolates found in the nasal cavities of piglets
Virginia Aragon
Institut de Recerca i Tecnologia Agroalimentaries (IRTA)
Corresponding Author
ORCiD: https://orcid.org/0000-0002-3470-6015
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Veterinary Research.
Background: Previous studies have shown that the genus Moraxella is commonly present in the nasal microbiota of swine.
Results: In this study, 51 isolates of Moraxella were obtained from nasal swabs from 3-4 week old piglets, which represented 26 different fingerprintings by enterobacterial repetitive intergenic consensus (ERIC)-PCR. Whole 16S rRNA gene sequencing allowed the identification at species level of the Moraxella spp. isolates. The majority of the field strains were identified as Moraxella pluranimalium, but Moraxella porci was also detected. In addition, a cluster of 7 strains did not group with any described Moraxella species, probably representing a new species. Subsequent phenotypic characterization indicated that strains of Moraxella pluranimalium were mainly sensitive to serum complement, while the cluster representing the putative new species was highly resistant. Biofilm formation capacity was very variable among the Moraxella spp. isolates, while adherence to epithelial cell lines was similar among selected strains. Additionally, variability was also observed in the association of selected strains to porcine alveolar macrophages. Antimicrobial tests evidenced the existence of multidrug-resistance in the strains.
Conclusions : In summary, phenotypic characterization revealed heterogeneity among Moraxella strains from the nasal cavity of piglets. Strains with pathogenic potential were detected as well as those that may be commensal members of the nasal microbiota. However, the role of Moraxella in porcine diseases and health should be further evaluated.
Keywords
Swine, Nasal microbiota, Virulence, Bacterial diversity, Moraxella
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CURRENT STATUS: Published
Version 2
Posted 31 Dec, 2019
Published
On 30 Jan, 2020
No community comments so far
Editorial decision: Minor revision
On 11 Jan, 2020
Review #2 received
Received 10 Jan, 2020
Review #1 received
Received 10 Jan, 2020
Reviewer #2 agreed
On 30 Dec, 2019
Reviewer #1 agreed
On 30 Dec, 2019
Reviewers invited
Invitations sent on 27 Dec, 2019
Editor assigned
On 22 Dec, 2019
Submission checks complete
On 21 Dec, 2019
Editor invited
On 21 Dec, 2019
No comments provided
Editorial decision: Major revision
On 13 Dec, 2019
Review #3 received
Received 12 Dec, 2019
Review #2 received
Received 02 Dec, 2019
Review #1 received
Received 25 Nov, 2019
Reviewer #3 agreed
On 19 Nov, 2019
Reviewer #2 agreed
On 18 Nov, 2019
Reviewers invited
Invitations sent on 12 Nov, 2019
Reviewer #1 agreed
On 12 Nov, 2019
Editor assigned
On 11 Nov, 2019
Submission checks complete
On 10 Nov, 2019
Editor invited
On 10 Nov, 2019
First submitted
On 06 Nov, 2019
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Subject Areas
Large Animal Medicine
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This preprint is under consideration at BMC Veterinary Research. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Heterogeneity of Moraxella isolates found in the nasal cavities of piglets
Virginia Aragon
Institut de Recerca i Tecnologia Agroalimentaries (IRTA)
Corresponding Author
ORCiD: https://orcid.org/0000-0002-3470-6015
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Version 2
Posted 31 Dec, 2019
Published
On 30 Jan, 2020
No community comments so far
Editorial decision: Minor revision
On 11 Jan, 2020
Review #2 received
Received 10 Jan, 2020
Review #1 received
Received 10 Jan, 2020
Reviewer #2 agreed
On 30 Dec, 2019
Reviewer #1 agreed
On 30 Dec, 2019
Reviewers invited
Invitations sent on 27 Dec, 2019
Editor assigned
On 22 Dec, 2019
Submission checks complete
On 21 Dec, 2019
Editor invited
On 21 Dec, 2019
No comments provided
Editorial decision: Major revision
On 13 Dec, 2019
Review #3 received
Received 12 Dec, 2019
Review #2 received
Received 02 Dec, 2019
Review #1 received
Received 25 Nov, 2019
Reviewer #3 agreed
On 19 Nov, 2019
Reviewer #2 agreed
On 18 Nov, 2019
Reviewers invited
Invitations sent on 12 Nov, 2019
Reviewer #1 agreed
On 12 Nov, 2019
Editor assigned
On 11 Nov, 2019
Submission checks complete
On 10 Nov, 2019
Editor invited
On 10 Nov, 2019
First submitted
On 06 Nov, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Large Animal Medicine
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Veterinary Research.
Background: Previous studies have shown that the genus Moraxella is commonly present in the nasal microbiota of swine.
Results: In this study, 51 isolates of Moraxella were obtained from nasal swabs from 3-4 week old piglets, which represented 26 different fingerprintings by enterobacterial repetitive intergenic consensus (ERIC)-PCR. Whole 16S rRNA gene sequencing allowed the identification at species level of the Moraxella spp. isolates. The majority of the field strains were identified as Moraxella pluranimalium, but Moraxella porci was also detected. In addition, a cluster of 7 strains did not group with any described Moraxella species, probably representing a new species. Subsequent phenotypic characterization indicated that strains of Moraxella pluranimalium were mainly sensitive to serum complement, while the cluster representing the putative new species was highly resistant. Biofilm formation capacity was very variable among the Moraxella spp. isolates, while adherence to epithelial cell lines was similar among selected strains. Additionally, variability was also observed in the association of selected strains to porcine alveolar macrophages. Antimicrobial tests evidenced the existence of multidrug-resistance in the strains.
Conclusions : In summary, phenotypic characterization revealed heterogeneity among Moraxella strains from the nasal cavity of piglets. Strains with pathogenic potential were detected as well as those that may be commensal members of the nasal microbiota. However, the role of Moraxella in porcine diseases and health should be further evaluated.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6