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Research article
H. Wang
Peking Univeristy People's Hospital
Corresponding Author
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Background: Acinetobacter baumannii colonizing the respiratory tract has been established as an independent risk factor for bacteremia. However, within-host evolution of A. baumannii in bacteremia has not been extensively investigated. Here we performed whole genome sequencing to discover the evolutionary characteristics that accompany the transition from respiratory tract carriage to bloodstream infection in three A. baumannii bacteremia patients.
Results: Within-host genetic diversity was identified. A total of twenty-one SNVs were detected. Genic and intergenic evolution occurred particularly in secretion system, DNA recombination and cell motility genes. Intergenic SNVs occurred more frequently than synonymous and non-synonymous SNVs, which indicated potential transcription or translation regulation. Non-synonymous mutations mostly occurred during the transition from the respiratory tract carriage to the bloodstream infection. Isolates of clonal complex 208 (CC208) had lower substitution rate with approximately 10-6 nucleotide substitutions per site year-1, compared with non CC208 isolates (approximately 10-5). We found evidence for the occurrence of recombination in one patient. Gene content showed patient specificity, and isolates within a single host had constrained gene content diversity.
Conclusions: Our results indicated that high within-host diversity was driven by rapid mutation rates and limited effect of recombination in A. baumannii from respiratory tract carriage to bacteremia.
Keywords
Acinetobacter baumannii, within-host evolution, mutation, recombination, bacteremia
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research article
H. Wang
Peking Univeristy People's Hospital
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 06 Oct, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Epigenetics & Genomics
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Acinetobacter baumannii colonizing the respiratory tract has been established as an independent risk factor for bacteremia. However, within-host evolution of A. baumannii in bacteremia has not been extensively investigated. Here we performed whole genome sequencing to discover the evolutionary characteristics that accompany the transition from respiratory tract carriage to bloodstream infection in three A. baumannii bacteremia patients.
Results: Within-host genetic diversity was identified. A total of twenty-one SNVs were detected. Genic and intergenic evolution occurred particularly in secretion system, DNA recombination and cell motility genes. Intergenic SNVs occurred more frequently than synonymous and non-synonymous SNVs, which indicated potential transcription or translation regulation. Non-synonymous mutations mostly occurred during the transition from the respiratory tract carriage to the bloodstream infection. Isolates of clonal complex 208 (CC208) had lower substitution rate with approximately 10-6 nucleotide substitutions per site year-1, compared with non CC208 isolates (approximately 10-5). We found evidence for the occurrence of recombination in one patient. Gene content showed patient specificity, and isolates within a single host had constrained gene content diversity.
Conclusions: Our results indicated that high within-host diversity was driven by rapid mutation rates and limited effect of recombination in A. baumannii from respiratory tract carriage to bacteremia.
Figure 1
Figure 2
Figure 3
Figure 4
This is a list of supplementary files associated with this preprint. Click to download.
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