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Improvement of Xylose Utilization and L-ornithine Production by Metabolic Engineering of Corynebacterium glutamicum
Ying Zhou
East China University of Science and Technology
Corresponding Author
ORCiD: https://orcid.org/0000-0001-9241-1293
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: L-ornithine is a basic amino acid, which shows significant value in food and medicine industries. Xylose is the most important alternative carbon source of glucose in lignocellulosic hydrolysate. It is urgent to develop a high-efficiency cell factory for L-ornithine production with glucose and xylose.
Results: In this study, the genes enconding xylose isomerase and xylulose kinase were introduced into Corynebacterium glutamicum S9114 to establish xylose metabolism pathway, and then xylose became a substitute carbon source of glucose. In addition, the optimization and overexpression of phosphoenolpyruvate carboxylase and pentose transporter had been conducted to promote the synthesis of L-ornithine for the first time. Furthermore, though optimizing the concentration ratio of glucose and xylose (7:3), adding biotin and thiamine hydrochloride, we arrived at the highest L-ornithine yield 41.5g/L in shaking flask fermentation so far.
Conclusions: Our results demonstrate that the combination of metabolic engineering and the optimization of fermentation process can make great potential for L-ornithine production by lignocellulose hydrolysate.
Keywords
L-ornithine, MCorynebacterium glutamicum, Xylose, Metabolic engineering
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This is a list of supplementary files associated with this preprint. Click to download.
- TableS1.pdf
Table S1. Primers involved in this article
- FigureS1.pdf
Fig. S1. Effect of different pH on L-ornithine yield. a Growth condition of C. glutamicum XAB03 at 72 h under different pH. b L‑Ornithine production of C. glutamicum XAB03 at 72 h under different pH.
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Research
Improvement of Xylose Utilization and L-ornithine Production by Metabolic Engineering of Corynebacterium glutamicum
Ying Zhou
East China University of Science and Technology
Corresponding Author
ORCiD: https://orcid.org/0000-0001-9241-1293
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 22 Sep, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Biotechnology and Bioengineering
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: L-ornithine is a basic amino acid, which shows significant value in food and medicine industries. Xylose is the most important alternative carbon source of glucose in lignocellulosic hydrolysate. It is urgent to develop a high-efficiency cell factory for L-ornithine production with glucose and xylose.
Results: In this study, the genes enconding xylose isomerase and xylulose kinase were introduced into Corynebacterium glutamicum S9114 to establish xylose metabolism pathway, and then xylose became a substitute carbon source of glucose. In addition, the optimization and overexpression of phosphoenolpyruvate carboxylase and pentose transporter had been conducted to promote the synthesis of L-ornithine for the first time. Furthermore, though optimizing the concentration ratio of glucose and xylose (7:3), adding biotin and thiamine hydrochloride, we arrived at the highest L-ornithine yield 41.5g/L in shaking flask fermentation so far.
Conclusions: Our results demonstrate that the combination of metabolic engineering and the optimization of fermentation process can make great potential for L-ornithine production by lignocellulose hydrolysate.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6