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Primary research
Quantitative Chemical Proteomics Reveals Resveratrol Inhibition of A549 Cell Migration Through Binding Multiple Targets to Regulate Cytoskeleton Remodeling and Suppress EMT
Jigang Wang
China Academy of Chinese Medical Sciences
Corresponding Author
ORCiD: https://orcid.org/0000-0002-0575-0105
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Resveratrol (RSV), a cancer chemopreventive natural product, has been shown to affect various cellular processes in tumor cells. However, RSV’s specific protein targets as well as its mechanism of action (MOA) of anticancer effect remain elusive. Recently, quantitative chemical proteomics approach has been an effective tool to identify target proteins, elucidate MOA and predict side effects for active small molecules, especially for natural products.
Methods
The pharmacological activity of RSV was evaluated in A549 cell lines, including the effects on cell proliferation and migration. To elucidate the underlying mechanism, a quantitative chemical proteomics approach was employed to identify the protein targets of RSV. With subsequent in vitro and in vivo experiments, RSV-related pathways and cellular processes were predicted and validated.
Results
RSV significantly inhibited A549 cell migration, but did not affect cell viability. With chemical proteomics approach, 38 target proteins were identified, and proteomic analysis showed that the targets were mainly involved in cytoskeleton remodeling and EMT, which were verified by subsequent in vitro and in vivo assays.
Conclusions
RSV inhibits A549 cell migration through binding multiple targets to regulate cytoskeleton remodeling and suppress EMT.
Keywords
Resveratrol, Quantitative chemical proteomics, Target identification, Cytoskeleton remodeling, EMT
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This is a preprint. It has not completed peer review.
Primary research
Quantitative Chemical Proteomics Reveals Resveratrol Inhibition of A549 Cell Migration Through Binding Multiple Targets to Regulate Cytoskeleton Remodeling and Suppress EMT
Jigang Wang
China Academy of Chinese Medical Sciences
Corresponding Author
ORCiD: https://orcid.org/0000-0002-0575-0105
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 23 Sep, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Cancer Biology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Resveratrol (RSV), a cancer chemopreventive natural product, has been shown to affect various cellular processes in tumor cells. However, RSV’s specific protein targets as well as its mechanism of action (MOA) of anticancer effect remain elusive. Recently, quantitative chemical proteomics approach has been an effective tool to identify target proteins, elucidate MOA and predict side effects for active small molecules, especially for natural products.
Methods
The pharmacological activity of RSV was evaluated in A549 cell lines, including the effects on cell proliferation and migration. To elucidate the underlying mechanism, a quantitative chemical proteomics approach was employed to identify the protein targets of RSV. With subsequent in vitro and in vivo experiments, RSV-related pathways and cellular processes were predicted and validated.
Results
RSV significantly inhibited A549 cell migration, but did not affect cell viability. With chemical proteomics approach, 38 target proteins were identified, and proteomic analysis showed that the targets were mainly involved in cytoskeleton remodeling and EMT, which were verified by subsequent in vitro and in vivo assays.
Conclusions
RSV inhibits A549 cell migration through binding multiple targets to regulate cytoskeleton remodeling and suppress EMT.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7