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Research article
Enhanced tolerance to Phytophthora root and stem rot by over-expression of the plant antimicrobial peptide CaAMP1 gene in soybean
Xiangdong Yang
Jilin Academy of Agricultural Sciences
Corresponding Author
ORCiD: https://orcid.org/0000-0002-7101-1626
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Genomic Data.
Background: Antimicrobial peptides play important roles in both plant and animal defense systems. Moreover, over-expression of CaAMP1 (Capsicum annuum antimicrobial protein 1), an antimicrobial protein gene isolated from C. annuum leaves infected with Xanthomonas campestris pv. vesicatoria, confers broad-spectrum resistance to hemibiotrophic bacterial and necrotrophic fungal pathogens in Arabidopsis. Phytophthora root and stem rot (PRR), caused by the fungus Phytophthora sojae, is one of the most devastating diseases affecting soybean (Glycine max) production worldwide.
Results: In this study, CaAMP1 was transformed into soybean by Agrobacterium-mediated genetic transformation. Integration of the foreign gene in the genome of transgenic soybean plants and its expression at the translation level were verified by Southern and western blot analyses, respectively. CaAMP1 over-expression (CaAMP1-OX) lines inoculated with P. sojae race 1 exhibited enhanced and stable PRR tolerance through T2–T4 generations compared with the wild-type Williams 82 plants. Gene expression analyses in the transgenic plants revealed that the expression of salicylic acid-dependent, jasmonic acid-dependent, and plant disease resistance (R-genes) genes were significantly up-regulated after P. sojae inoculation.
Conclusions: These results indicate that CaAMP1 over-expression can significantly enhance PRR tolerance in soybean by eliciting resistance responses mediated by multiple defense signaling pathways. This provides an alternative approach for developing soybean varieties with improved tolerance against soil-borne pathogenic PRR.
Keywords
transgenic soybean, PRR tolerance, antimicrobial peptide
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CURRENT STATUS: Published
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Posted 17 Jun, 2020
Published
On 06 Jul, 2020
No community comments so far
Editorial decision: Accept
On 18 Jun, 2020
Editor assigned
On 16 Jun, 2020
Submission checks complete
On 15 Jun, 2020
Editor invited
On 15 Jun, 2020
No comments provided
Editorial decision: Minor revision
On 04 Jun, 2020
Review #2 received
Received 21 Apr, 2020
Reviewer #2 agreed
On 01 Apr, 2020
Review #1 received
Received 23 Dec, 2019
Reviewers invited
Invitations sent on 10 Dec, 2019
Reviewer #1 agreed
On 10 Dec, 2019
Editor invited
On 20 Nov, 2019
First submitted
On 14 Nov, 2019
Editor assigned
On 14 Nov, 2019
Submission checks complete
On 13 Nov, 2019
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Subject Areas
Population Genetics
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This preprint is under consideration at BMC Genomic Data. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Enhanced tolerance to Phytophthora root and stem rot by over-expression of the plant antimicrobial peptide CaAMP1 gene in soybean
Xiangdong Yang
Jilin Academy of Agricultural Sciences
Corresponding Author
ORCiD: https://orcid.org/0000-0002-7101-1626
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Version 2
Posted 17 Jun, 2020
Published
On 06 Jul, 2020
No community comments so far
Editorial decision: Accept
On 18 Jun, 2020
Editor assigned
On 16 Jun, 2020
Submission checks complete
On 15 Jun, 2020
Editor invited
On 15 Jun, 2020
No comments provided
Editorial decision: Minor revision
On 04 Jun, 2020
Review #2 received
Received 21 Apr, 2020
Reviewer #2 agreed
On 01 Apr, 2020
Review #1 received
Received 23 Dec, 2019
Reviewers invited
Invitations sent on 10 Dec, 2019
Reviewer #1 agreed
On 10 Dec, 2019
Editor invited
On 20 Nov, 2019
First submitted
On 14 Nov, 2019
Editor assigned
On 14 Nov, 2019
Submission checks complete
On 13 Nov, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Population Genetics
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Genomic Data.
Background: Antimicrobial peptides play important roles in both plant and animal defense systems. Moreover, over-expression of CaAMP1 (Capsicum annuum antimicrobial protein 1), an antimicrobial protein gene isolated from C. annuum leaves infected with Xanthomonas campestris pv. vesicatoria, confers broad-spectrum resistance to hemibiotrophic bacterial and necrotrophic fungal pathogens in Arabidopsis. Phytophthora root and stem rot (PRR), caused by the fungus Phytophthora sojae, is one of the most devastating diseases affecting soybean (Glycine max) production worldwide.
Results: In this study, CaAMP1 was transformed into soybean by Agrobacterium-mediated genetic transformation. Integration of the foreign gene in the genome of transgenic soybean plants and its expression at the translation level were verified by Southern and western blot analyses, respectively. CaAMP1 over-expression (CaAMP1-OX) lines inoculated with P. sojae race 1 exhibited enhanced and stable PRR tolerance through T2–T4 generations compared with the wild-type Williams 82 plants. Gene expression analyses in the transgenic plants revealed that the expression of salicylic acid-dependent, jasmonic acid-dependent, and plant disease resistance (R-genes) genes were significantly up-regulated after P. sojae inoculation.
Conclusions: These results indicate that CaAMP1 over-expression can significantly enhance PRR tolerance in soybean by eliciting resistance responses mediated by multiple defense signaling pathways. This provides an alternative approach for developing soybean varieties with improved tolerance against soil-borne pathogenic PRR.
Figure 1
Figure 2
Figure 3
Figure 4