In this work, we are reporting headless mutant in Zaprionus indianus in month of February from (Punjab), India in 2021 for the first time.
Short Report
First record of headless mutant in Zaprionus indianus (Diptera: Drosophilidae)
https://doi.org/10.21203/rs.3.rs-842062/v1
This work is licensed under a CC BY 4.0 License
posted 26 Aug, 2021
You are reading this latest preprint version
In this work, we are reporting headless mutant in Zaprionus indianus in month of February from (Punjab), India in 2021 for the first time.
Zaprionus indianus
Head less mutant
Several morphological mutants are evidenced in Drosophila, which are commonly used to know the proper function of any gene. Mutations are neither good nor bad: some can be beneficial to the body; others can be fatal. With the creation of new versions of genes, mutations are the driving force behind the evolutionary changes that sometimes lead to new species. Headless flies produced by mutations in the paralogous Pax6 genes eyeless and twin of eyeless ( Kronhamn et al., 2002) Moderate levels of Ey protein are adequate to rescue the headless phenotype, whereas high level of eyeless protein are required to rescue the eyeless phenotype. Ey protein required to inhibit cell death and to promote eye development whereas Toy protein is required for the activation of the Ey gene, Headless mutants are results of strong mutant of twin of eyeless or of eyeless (Kronhamn et al., 2002). Zaprionus indianus native to Africa and was first recorded from India (Gupta, 1970). In this work, we are reporting headless mutant in Zaprionus indianus in month of February from (Punjab), India in 2021 for the first time (Fig. 1). The wild collected flies showed normal phenotype as well as three headless mutant male only.
We took the stereo zoom microscopic image of headless mutant fly to check the modified head region (Fig. 2).
Simultaneously, we took the scanning electron microscopic image of normal and mutant Zaprionus indianus (Fig. 3) for clear observations.
The headless mutants were able to survive in laboratory condition for 24 hours only after that they died. When we reared the wild caught flies of Zaprionus indianus at 25°C in laboratory conditions than 100% of them were of normal phenotype. These headless mutants can be used for comparison of developmental studies in nature of normal phenotype and mutant phenotypes.
The authors declare no competing interests.
Kronhamn, J., Frei, E., Daube, M., Jiao, R., Shi, Y., Noll, M. and Rasmuson-Lestander, A., 2002. Headless flies produced by mutations in the paralogous Pax6 genes eyeless and twin of eyeless. Gupta, J.P., 1970. Description of a new species of Phorticella zaprionus (Drosophilidae) from India. Nat Inst Sci India Proc. Costa, L.V.C., S.C. Costa, J.O. Rios, M.T.R. Cintra, and L.G.C. Galego., 2018. First record of Curly mutant wing in Zaprionus indianus ((Diptera Drosophilidae). Dros. Inf. Serv. 101: 75
posted 26 Aug, 2021
You are reading this latest preprint version