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Research article
Peng Cai
Huazhong Agriculture University
Corresponding Author
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This work is licensed under a CC BY 4.0 License. Read Full License
Background Bacterial biofilms are a surface-adherent microbial community in which individual cells are surrounded by a self-produced extracellular matrix of polysaccharide, extracellular DNA (eDNA) and proteins. Interactions among matrix components within the biofilms are responsible for creating an adaptable structure during biofilm development. However, it is unclear how the interaction among matrix components contributes to the construction of the three-dimensional (3D) biofilm architecture.
Results DNase I treatment could significantly inhibit B. subtilis biofilm formation in early phases. Confocal laser scanning microscopy (CLSM) and image analysis revealed that eDNA was cooperative with exopolysaccharide (EPS) in early stages of B. subtilis biofilm development, while EPS played a major structural role in the later stages. In addition, deletion of EPS production gene epsG in B. subtilis SBE1 resulted in loss of the interaction between EPS and eDNA, and reduction of biofilm biomass in pellicles at air-liquid interface. The physical interaction between these two essential biofilm matrix components was confirmed by isothermal titration calorimetry (ITC).
Conclusions The biofilm 3D structures become interconnected through surrounding eDNA and EPS. eDNA interacts with EPS in the early phases of biofilm development, while EPS mainly participates in the maturation of biofilm. The findings of this study provide better understanding of the role of interaction between eDNA and EPS in shaping the biofilm 3D matrix structure and biofilm formation.
Keywords
extracellular DNA (eDNA), exopolysaccharide (EPS), Bacillus subtilis, biofilm formation
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CURRENT STATUS: Published
View the published article at BMC Microbiology.
Version 3
Posted 23 Apr, 2020
No community comments so far
Submission checks complete
On 16 Apr, 2020
Editorial decision: Accept
On 16 Apr, 2020
No comments provided
Editorial decision: Minor revision
On 07 Apr, 2020
Editor assigned
On 29 Mar, 2020
Submission checks complete
On 28 Mar, 2020
Editor invited
On 28 Mar, 2020
No comments provided
Editorial decision: Major revision
On 28 Feb, 2020
Review #2 received
Received 22 Feb, 2020
Reviewer #2 agreed
On 04 Feb, 2020
Review #1 received
Received 02 Jan, 2020
Reviewer #1 agreed
On 10 Dec, 2019
Editor assigned
On 04 Dec, 2019
Reviewers invited
Invitations sent on 04 Dec, 2019
Submission checks complete
On 03 Dec, 2019
Editor invited
On 02 Dec, 2019
First submitted
On 28 Nov, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
General Microbiology
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A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
See the published version of this preprint at BMC Microbiology.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Peng Cai
Huazhong Agriculture University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Microbiology.
Version 3
Posted 23 Apr, 2020
No community comments so far
Submission checks complete
On 16 Apr, 2020
Editorial decision: Accept
On 16 Apr, 2020
No comments provided
Editorial decision: Minor revision
On 07 Apr, 2020
Editor assigned
On 29 Mar, 2020
Submission checks complete
On 28 Mar, 2020
Editor invited
On 28 Mar, 2020
No comments provided
Editorial decision: Major revision
On 28 Feb, 2020
Review #2 received
Received 22 Feb, 2020
Reviewer #2 agreed
On 04 Feb, 2020
Review #1 received
Received 02 Jan, 2020
Reviewer #1 agreed
On 10 Dec, 2019
Editor assigned
On 04 Dec, 2019
Reviewers invited
Invitations sent on 04 Dec, 2019
Submission checks complete
On 03 Dec, 2019
Editor invited
On 02 Dec, 2019
First submitted
On 28 Nov, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
General Microbiology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Microbiology.
Background Bacterial biofilms are a surface-adherent microbial community in which individual cells are surrounded by a self-produced extracellular matrix of polysaccharide, extracellular DNA (eDNA) and proteins. Interactions among matrix components within the biofilms are responsible for creating an adaptable structure during biofilm development. However, it is unclear how the interaction among matrix components contributes to the construction of the three-dimensional (3D) biofilm architecture.
Results DNase I treatment could significantly inhibit B. subtilis biofilm formation in early phases. Confocal laser scanning microscopy (CLSM) and image analysis revealed that eDNA was cooperative with exopolysaccharide (EPS) in early stages of B. subtilis biofilm development, while EPS played a major structural role in the later stages. In addition, deletion of EPS production gene epsG in B. subtilis SBE1 resulted in loss of the interaction between EPS and eDNA, and reduction of biofilm biomass in pellicles at air-liquid interface. The physical interaction between these two essential biofilm matrix components was confirmed by isothermal titration calorimetry (ITC).
Conclusions The biofilm 3D structures become interconnected through surrounding eDNA and EPS. eDNA interacts with EPS in the early phases of biofilm development, while EPS mainly participates in the maturation of biofilm. The findings of this study provide better understanding of the role of interaction between eDNA and EPS in shaping the biofilm 3D matrix structure and biofilm formation.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
This is a list of supplementary files associated with this preprint. Click to download.
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