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Research article
Yellow nutsedge WRI3/4-like gene improves drought tolerance in Arabidopsis thaliana by promoting cuticular wax biosynthesis
Bangquan Huang
Hubei University
Corresponding Author
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This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Plant Biology.
Using RACE PCR, full length WRI1-like gene was amplified from yellow nutsedge. Conserved domain and phylogenetic analyses suggested it as WRI3/4-like gene. Tissue-specific expression data showed the highest expression in leaves, followed by roots while the lowest expression was detected in tuber. Transgenic Arabidopsis plants expressing nutsedge WRI3/4-like gene showed significantly improved tolerance to both PEG-simulated drought stress and real dehydration, compared with the wild type (WT). Under normal growth conditions, the expressions of key fatty acid biosynthesis genes was not significantly different between WT and transgenic lines, while the expressions of genes involved in cuticular wax biosynthesis was significantly higher in transgenic lines compared with the WT. The PEG-simulated drought stress did not induce any significant change in the expression of fatty acid and wax biosynthesis genes in WT plants, while the expression of fatty acid and wax biosynthesis genes was significantly increased in transgenic lines compared with WT as well as unstressed transgenic control. The expression of TAG1, the gene involved in triacylglycerol (TAG) accumulation, was significantly lower in the transgenic lines than that in the WT in normal growth conditions. Drought stress slightly decreased the expression of TAG1 in the WT, but significantly lowered it in transgenic lines compared with its unstressed transgenic control and WT. Consistent with gene expression data, the cuticular wax content in Arabidopsis leaves was significantly higher in the transgenic lines than in the WT, while the oil content was not significantly different. Our results indicated that WRI3/4-like gene from Cyperus esculentus improves drought tolerance in Arabidopsis probably by promoting cuticular wax biosynthesis and, hence, could be a valuable target for improving drought tolerance in crops through recombinant DNA technology.
Keywords
Cyperus esculentus, WRI3/4-like gene, cuticular wax biosynthesis, drought tolerance, gene expression
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This preprint is under consideration at BMC Plant Biology. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Yellow nutsedge WRI3/4-like gene improves drought tolerance in Arabidopsis thaliana by promoting cuticular wax biosynthesis
Bangquan Huang
Hubei University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Version 2
Posted 19 Dec, 2019
Published
On 31 Oct, 2020
No community comments so far
Review #2 received
Received 25 Apr, 2020
Reviewer #2 agreed
On 26 Mar, 2020
Review #1 received
Received 03 Mar, 2020
Reviewer #1 agreed
On 18 Feb, 2020
Reviewers invited
Invitations sent on 02 Jan, 2020
Editor assigned
On 17 Dec, 2019
Submission checks complete
On 16 Dec, 2019
Editor invited
On 16 Dec, 2019
No comments provided
Editorial decision: Revise before sending to peer reviewers
On 03 Dec, 2019
Editor assigned
On 27 Nov, 2019
Submission checks complete
On 26 Nov, 2019
Editor invited
On 26 Nov, 2019
First submitted
On 22 Nov, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Plant Biology.
Using RACE PCR, full length WRI1-like gene was amplified from yellow nutsedge. Conserved domain and phylogenetic analyses suggested it as WRI3/4-like gene. Tissue-specific expression data showed the highest expression in leaves, followed by roots while the lowest expression was detected in tuber. Transgenic Arabidopsis plants expressing nutsedge WRI3/4-like gene showed significantly improved tolerance to both PEG-simulated drought stress and real dehydration, compared with the wild type (WT). Under normal growth conditions, the expressions of key fatty acid biosynthesis genes was not significantly different between WT and transgenic lines, while the expressions of genes involved in cuticular wax biosynthesis was significantly higher in transgenic lines compared with the WT. The PEG-simulated drought stress did not induce any significant change in the expression of fatty acid and wax biosynthesis genes in WT plants, while the expression of fatty acid and wax biosynthesis genes was significantly increased in transgenic lines compared with WT as well as unstressed transgenic control. The expression of TAG1, the gene involved in triacylglycerol (TAG) accumulation, was significantly lower in the transgenic lines than that in the WT in normal growth conditions. Drought stress slightly decreased the expression of TAG1 in the WT, but significantly lowered it in transgenic lines compared with its unstressed transgenic control and WT. Consistent with gene expression data, the cuticular wax content in Arabidopsis leaves was significantly higher in the transgenic lines than in the WT, while the oil content was not significantly different. Our results indicated that WRI3/4-like gene from Cyperus esculentus improves drought tolerance in Arabidopsis probably by promoting cuticular wax biosynthesis and, hence, could be a valuable target for improving drought tolerance in crops through recombinant DNA technology.
Figure 1
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Figure 3
Figure 4
Figure 5
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Figure 8