Turnover among teaching staff in our universities are mostly due to dissatisfaction with their jobs. Employee job satisfaction is of utmost importance to stimulating and sustaining the interest of the individual in order to prevent employee turnover. There are many factors influencing employee job satisfaction and preventing employee turnover. Factors such as salary, working conditions, cordial relationship with colleagues at work, opportunities for job progression among several other factors. The objective of this paper was to explore the extent to which these factors, affect employee job satisfaction and prevent turnover among lecturers in the University for Development Studies using the cross-sectional design and quantitative approach of data collection. Survey questionnaires were employed as data collection instruments to elicit information from 287 lecturers recruited from the University for Development Studies within the Tamale Metropolis. Multiple regression was used to establish the effect factors influencing job satisfaction had on employee turnover. Major findings from the study alluded to the fact that salary, working conditions, working environments, carrier advancement, relationship with co-workers have significant correlation with job satisfaction and employee turnover of lecturers in this institution. On account of these findings, this paper suggests yearly salary adjustments, creates a conducive working environment for lecturers, improve upon infrastructural facilities and facilitates carrier advancement issues for lecturers so as to prevent them from exiting the institution.