This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research article
Peng Yuan
Fourth Military Medical University: Air Force Medical University
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Osteosarcoma (OS) is one of the most aggressive malignant bone tumor worldwide. This study focuses on investigating the mechanism underlying the mitochondrial calcium uniporter regulator 1 (MCUR1)-mediated osteosarcoma (OS) cell growth.
Methods: A total of 49 patients diagnosed as OS in our hospital were included in the current study. The expression of MCUR1 in human OS tissues and various OS cell lines was detected by immunohistochemical staining (IHC) and western blot analysis, respectively. The effect of MCUR1 on AKT/p53 pathway and its correlation with miR-506-3p were investigated by a series of experiments including MTS, QRT-PCR, Western blot and IHC.
Results: Our data showed that MCUR1 was highly overexpressed in OS tumor tissues compared with the para-carcinoma tissues (P<0.01). The Kaplan-Meier analysis showed that high expression level of MCUR1 was linked with poor prognosis of OS patients. Additionally, knockdown of MCUR1 enhanced OS cell apoptosis and decreased the growth of OS cells compared with the corresponding controls (P<0.05). Meanwhile, the expression level of p-AKT was decreased, whereas the protein expression level of p53 was increased in OS cells with MCUR1 downregulation. We also found that the IHC scores of MCUR1 were inversely correlated with that of p53 (r = -0.304, P=0.034). Likewise, the expression of MCUR1 and miR-506-3p in OS tissues were also inversely correlated (r=-0.304, P=0.034).
Conclusions: MCUR1 is overexpressed in OS cells and its expression is regulated by miR-506-3p. MCUR1 facilitates the progression of OS through activating AKT/p53 pathway. The data in the current study suggests that MCUR1 may serve as a new target for the diagnosis and treatment of OS.
Keywords
Osteosarcoma, MCUR1, prognosis, apoptosis, p53
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research article
Peng Yuan
Fourth Military Medical University: Air Force Medical University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 27 Sep, 2021
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Orthopedic Surgery
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Osteosarcoma (OS) is one of the most aggressive malignant bone tumor worldwide. This study focuses on investigating the mechanism underlying the mitochondrial calcium uniporter regulator 1 (MCUR1)-mediated osteosarcoma (OS) cell growth.
Methods: A total of 49 patients diagnosed as OS in our hospital were included in the current study. The expression of MCUR1 in human OS tissues and various OS cell lines was detected by immunohistochemical staining (IHC) and western blot analysis, respectively. The effect of MCUR1 on AKT/p53 pathway and its correlation with miR-506-3p were investigated by a series of experiments including MTS, QRT-PCR, Western blot and IHC.
Results: Our data showed that MCUR1 was highly overexpressed in OS tumor tissues compared with the para-carcinoma tissues (P<0.01). The Kaplan-Meier analysis showed that high expression level of MCUR1 was linked with poor prognosis of OS patients. Additionally, knockdown of MCUR1 enhanced OS cell apoptosis and decreased the growth of OS cells compared with the corresponding controls (P<0.05). Meanwhile, the expression level of p-AKT was decreased, whereas the protein expression level of p53 was increased in OS cells with MCUR1 downregulation. We also found that the IHC scores of MCUR1 were inversely correlated with that of p53 (r = -0.304, P=0.034). Likewise, the expression of MCUR1 and miR-506-3p in OS tissues were also inversely correlated (r=-0.304, P=0.034).
Conclusions: MCUR1 is overexpressed in OS cells and its expression is regulated by miR-506-3p. MCUR1 facilitates the progression of OS through activating AKT/p53 pathway. The data in the current study suggests that MCUR1 may serve as a new target for the diagnosis and treatment of OS.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
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