7–8 months old seedlings were collected from Kalimpong Forest Division West Bengal. The seedling was headed back and resulted stump was raised in polybags. Nodal segments were collected after 7-months old coppice shoot. These nodal segments were surfaced sterilized in 75 % (V/V) ethanol for 2 minutes. These segments were washed in Tween 20 for 25 minutes followed by washing three times in autoclaved deionized water. The washed nodal segments were further treated with 0.2 % bavistin for 20 minutes. Surface sterilization in laminar air flow was achieved using 0.1 per cent mercuric chloride for 8 minutes. Nodal segments were inoculated in large size culture tubes, containing previously autoclaved inoculation medium with containing MS basal salts and vitamins (Murashige and Skoog 1962) + 3.0 % sucrose (w/v) + 6.7 g L-1 agar + 1.00 mg L-1 BAP, with a pH value of 5.8. After 3 weeks, 3.0–4.0 cm long sections of in-vitro regenerated micro shoots were transferred into shoot proliferation media consisting of MS (with basal salt, vitamins, sucrose and agar) supplemented with hormone combinations viz. 0.25 mg L-1 BAP, 0.50 mg L-1 BAP, 1.00 mg L-1 BAP, 0.50 mg L-1 BAP + 0.10 mg L-1 NAA, 0.50 mg L-1 BAP + 0.10 mg L-1 IBA, 0.50 mg L-1 BAP + 0.10 mg L-1 IAA, 1.00 mg L-1 BAP + 0.10 mg L-1 NAA, 1.00 mg L-1 BAP + 0.10 mg L-1 IBA and 1.00 mg L-1 BAP + 0.10 mg L-1 IAA. A control (MS without hormone) was also maintained for comparison. Proliferated micro-shoots were cut into 2.5 to 3.0 cm sizes and transferred to a rooting medium consisting of ½ MS (half basal salts and vitamins) + 3.0 % sucrose (W/V) + 6.7 g L-1 agar, supplemented with 0.25 mg L-1 IBA, 0.50 mg L-1 IBA, 1.00 mg L-1 IBA, 0.25 mg L-1 NAA, 0.50 mg L-1 NAA, 0.50 mg L-1 IBA + 0.50 mg L-1 NAA and a control. The culture vessels were kept in an incubation room at 25±10C ambient temperature and 47µ mol m-2 S-1 fluorescent white light with 16 hours light and 8 hours dark cycle. Plantlets were put in root trainers containing sterilized vermiculite and were acclimatized in mist chamber with 80 % relative humidity and 25˚ ± 3˚C temperature. Observations on sprouting percentage during ex-plant inoculation, number of shoots explants-1, mean shoot length during shoot proliferation and number of roots per micro-shoots, rooting percentage and mean root length during rooting were recorded. Completely randomized block design having four replications with three micro-shoots in each replication were used for analysis. Treatment means were compared with the help of least significant difference at 5% level of significance.