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Research note
Mehru Nisha
Universiti Kuala Lumpur
Corresponding Author
ORCiD: https://orcid.org/0000-0003-2083-4267
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This work is licensed under a CC BY 4.0 License. Read Full License
Objective: Ascaris lumbricoides eggs are naturally available in contaminated soil and embryonated eggs are the source of Ascariasis infection to human. Investigation of in vitro culturing of Ascaris eggs in laboratory in not routinely reported in the literature. Hence, this study was carried out to investigate a simple method on in vitro culturing method for Ascaris lumbricoides eggs isolated from soil samples from an indigenous village in Malaysia.
Results: Four stages of Ascaris lumbricoides eggs from the first-cell stage until the fourth stage were obtained. At the embryonation stage, the larvae of Ascaris worm was observed indicating survival of the eggs under an acidic environment.
Keywords
Ascaris lumbricoides, Sulphuric acid, In vitro culture
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CURRENT STATUS: Under Review
Version 2
Posted 19 Mar, 2021
No community comments so far
Editorial decision: Reject after review
On 01 Mar, 2021
Review #1 received
Received 27 Feb, 2021
Reviewer #1 agreed
On 15 Feb, 2021
Editor assigned
On 10 Feb, 2021
Reviewers invited
Invitations sent on 10 Feb, 2021
Submission checks complete
On 10 Feb, 2021
Editor invited
On 10 Feb, 2021
First submitted
On 31 Dec, 2020
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This preprint is under consideration at BMC Research Notes. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research note
Mehru Nisha
Universiti Kuala Lumpur
Corresponding Author
ORCiD: https://orcid.org/0000-0003-2083-4267
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 2
Posted 19 Mar, 2021
No community comments so far
Editorial decision: Reject after review
On 01 Mar, 2021
Review #1 received
Received 27 Feb, 2021
Reviewer #1 agreed
On 15 Feb, 2021
Editor assigned
On 10 Feb, 2021
Reviewers invited
Invitations sent on 10 Feb, 2021
Submission checks complete
On 10 Feb, 2021
Editor invited
On 10 Feb, 2021
First submitted
On 31 Dec, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Objective: Ascaris lumbricoides eggs are naturally available in contaminated soil and embryonated eggs are the source of Ascariasis infection to human. Investigation of in vitro culturing of Ascaris eggs in laboratory in not routinely reported in the literature. Hence, this study was carried out to investigate a simple method on in vitro culturing method for Ascaris lumbricoides eggs isolated from soil samples from an indigenous village in Malaysia.
Results: Four stages of Ascaris lumbricoides eggs from the first-cell stage until the fourth stage were obtained. At the embryonation stage, the larvae of Ascaris worm was observed indicating survival of the eggs under an acidic environment.
Figure 1
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