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Takaaki Shimohata
Institute of Biomedical Sciences Tokushima University Graduate School
Corresponding Author
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Background: The prevalence of extended spectrum β-lactamase (ESBL)-producing Escherichia coli is increasing rapidlyand spreading worldwide, particularly in Asia compared to other regions. In the last 10 years, in our hospital, in particular, there has been <30% increase. To prevent the spread of ESBL in hospitals and in the community, the ultraviolet (UV) A-light-emitting diode (LED) irradiation device was used to inactivate ESBL- E. coli in human livestock and the environment .
Methods: ESBL- E. coli and E. coli bacterial samples were collected from patients at Tokushima University Hospital (Tokushima City, Japan). The UVA-LED irradiation system had 365 nm single wavelength, and the current of the circuit was set to 0.23 or 0.50 A consistently.
Results: Results demonstrated that UVA-LED was useful for the inactivation of ESBL- E. coli and E. coli . The minimum energy dosage required to inactivate ESBL- E. coli and E. coli was 40.75 J/cm 2 (45 min) in the first type of UVA-LED and 38.85 J/cm 2 (5 min) in the second type. There were no significant differences between ESBL- E. coli and E. coli . The inactivation of ESBL- E. coli was dependent on energy.
Conclusions: These findings suggest that UVA-LED with 365 nm single wavelength could be useful for surface decontamination in healthcare facilities.
Keywords
extended spectrum b-lactamase (ESBL, ESBL- E. coli, E. coli, UVA-LED irradiation
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A new preprint design is coming!
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research
Takaaki Shimohata
Institute of Biomedical Sciences Tokushima University Graduate School
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
The most recent version of this article is available here.
No community comments so far
Version 1
Posted 16 Dec, 2019
No comments provided
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Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
The most recent version of this article is available here.
Background: The prevalence of extended spectrum β-lactamase (ESBL)-producing Escherichia coli is increasing rapidlyand spreading worldwide, particularly in Asia compared to other regions. In the last 10 years, in our hospital, in particular, there has been <30% increase. To prevent the spread of ESBL in hospitals and in the community, the ultraviolet (UV) A-light-emitting diode (LED) irradiation device was used to inactivate ESBL- E. coli in human livestock and the environment .
Methods: ESBL- E. coli and E. coli bacterial samples were collected from patients at Tokushima University Hospital (Tokushima City, Japan). The UVA-LED irradiation system had 365 nm single wavelength, and the current of the circuit was set to 0.23 or 0.50 A consistently.
Results: Results demonstrated that UVA-LED was useful for the inactivation of ESBL- E. coli and E. coli . The minimum energy dosage required to inactivate ESBL- E. coli and E. coli was 40.75 J/cm 2 (45 min) in the first type of UVA-LED and 38.85 J/cm 2 (5 min) in the second type. There were no significant differences between ESBL- E. coli and E. coli . The inactivation of ESBL- E. coli was dependent on energy.
Conclusions: These findings suggest that UVA-LED with 365 nm single wavelength could be useful for surface decontamination in healthcare facilities.
Figure 1
Figure 2
Figure 3
Figure 4
This is a list of supplementary files associated with this preprint. Click to download.
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