See the published version of this preprint at Biotechnology for Biofuels and Bioproducts.
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Research
Chen-Guang Liu
Shanghai Jiao Tong University
Corresponding Author
ORCiD: https://orcid.org/0000-0003-0343-6304
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Microbial fuel cell (MFC) convokes microorganism as workhorse to convert biomass into electricity. However, most well-known electrogenic strains can not directly use glucose to produce valuable products. Zymomonas mobilis , a promising bacterial for ethanol production, owns special Entner-Doudoroff pathway with less ATP and biomass produced and the low energy-coupling respiration, making Z. mobilis a potential exoelectrogen.
Results
A glucose-consumed MFC is constructed by inoculating Z. mobilis . The electricity with power density 2.0 mW m -2 is derived from the difference of oxidation-reduction potential (ORP) between anode and cathode chambers. Besides, two-type electricity generation is observed as glucose-independent process and glucose-dependent process. For the sake of enhancing MFC efficiency, extracellular and intracellular strategies are implemented. Biofilm removal and addition of c -type cytochrome benefits electricity performance and Tween 80 accelerates the electricity generation. Perturbation of cellular redox balance compromises the electricity output, indicating that redox homeostasis is the principal requirement to reach idea voltage.
Conclusion
This study identifies potential feature of electricity activity for Z. mobilis and provides multiple strategies to enhance the electricity output. Therefore, additional electricity generation will benefit the techno-economic viability of the commercial bulk production for biochemicals or biofuels in an efficient and environmentally sustainable manner.
Keywords
Zymomonas mobilis; Microbial fuel cell (MFC); Biofilm; Extracellular electron transfer; Redox balance
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CURRENT STATUS: Published
View the published article at Biotechnology for Biofuels and Bioproducts.
No community comments so far
Editorial decision: Accept
On 31 Jan, 2020
Review #3 received
Received 27 Jan, 2020
Reviewer #3 agreed
On 25 Jan, 2020
Review #1 received
Received 25 Jan, 2020
Review #2 received
Received 25 Jan, 2020
Reviewers invited
Invitations sent on 24 Jan, 2020
Reviewer #1 agreed
On 24 Jan, 2020
Reviewer #2 agreed
On 24 Jan, 2020
Editor assigned
On 21 Jan, 2020
Submission checks complete
On 20 Jan, 2020
Editor invited
On 20 Jan, 2020
Version 1
Posted 16 Dec, 2019
No comments provided
Editorial decision: Major revision
On 06 Jan, 2020
Review #3 received
Received 05 Jan, 2020
Reviewer #3 agreed
On 22 Dec, 2019
Review #1 received
Received 19 Dec, 2019
Review #2 received
Received 19 Dec, 2019
Reviewer #2 agreed
On 17 Dec, 2019
Reviewers invited
Invitations sent on 17 Dec, 2019
Reviewer #1 agreed
On 17 Dec, 2019
Editor assigned
On 11 Dec, 2019
First submitted
On 10 Dec, 2019
Submission checks complete
On 10 Dec, 2019
Editor invited
On 10 Dec, 2019
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Subject Areas
Biotechnology and Bioengineering
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A new preprint design is coming!
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See the published version of this preprint at Biotechnology for Biofuels and Bioproducts.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Chen-Guang Liu
Shanghai Jiao Tong University
Corresponding Author
ORCiD: https://orcid.org/0000-0003-0343-6304
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at Biotechnology for Biofuels and Bioproducts.
No community comments so far
Editorial decision: Accept
On 31 Jan, 2020
Review #3 received
Received 27 Jan, 2020
Reviewer #3 agreed
On 25 Jan, 2020
Review #1 received
Received 25 Jan, 2020
Review #2 received
Received 25 Jan, 2020
Reviewers invited
Invitations sent on 24 Jan, 2020
Reviewer #1 agreed
On 24 Jan, 2020
Reviewer #2 agreed
On 24 Jan, 2020
Editor assigned
On 21 Jan, 2020
Submission checks complete
On 20 Jan, 2020
Editor invited
On 20 Jan, 2020
Version 1
Posted 16 Dec, 2019
No comments provided
Editorial decision: Major revision
On 06 Jan, 2020
Review #3 received
Received 05 Jan, 2020
Reviewer #3 agreed
On 22 Dec, 2019
Review #1 received
Received 19 Dec, 2019
Review #2 received
Received 19 Dec, 2019
Reviewer #2 agreed
On 17 Dec, 2019
Reviewers invited
Invitations sent on 17 Dec, 2019
Reviewer #1 agreed
On 17 Dec, 2019
Editor assigned
On 11 Dec, 2019
First submitted
On 10 Dec, 2019
Submission checks complete
On 10 Dec, 2019
Editor invited
On 10 Dec, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Biotechnology and Bioengineering
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Biotechnology for Biofuels and Bioproducts.
Background
Microbial fuel cell (MFC) convokes microorganism as workhorse to convert biomass into electricity. However, most well-known electrogenic strains can not directly use glucose to produce valuable products. Zymomonas mobilis , a promising bacterial for ethanol production, owns special Entner-Doudoroff pathway with less ATP and biomass produced and the low energy-coupling respiration, making Z. mobilis a potential exoelectrogen.
Results
A glucose-consumed MFC is constructed by inoculating Z. mobilis . The electricity with power density 2.0 mW m -2 is derived from the difference of oxidation-reduction potential (ORP) between anode and cathode chambers. Besides, two-type electricity generation is observed as glucose-independent process and glucose-dependent process. For the sake of enhancing MFC efficiency, extracellular and intracellular strategies are implemented. Biofilm removal and addition of c -type cytochrome benefits electricity performance and Tween 80 accelerates the electricity generation. Perturbation of cellular redox balance compromises the electricity output, indicating that redox homeostasis is the principal requirement to reach idea voltage.
Conclusion
This study identifies potential feature of electricity activity for Z. mobilis and provides multiple strategies to enhance the electricity output. Therefore, additional electricity generation will benefit the techno-economic viability of the commercial bulk production for biochemicals or biofuels in an efficient and environmentally sustainable manner.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10
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