Scaffolds with gradient porosity have become very promising candidates for tissue engineering and bone implants because of the combination of better mechanical and biological requirements. In this paper, a novel approach is proposed to design bone scaffolds with gradient porosity similar to the structure of cortical and spongy (cancellous) bones. The radial gradient PLA scaffolds were designed to consist of three different regions with the gyroid infill and, fabricated by Fused deposition modeling (FDM). The scaffolds with radial gradient porosity (G100-40-20) gave highest cell proliferation. Because, mean pore size is an important aspect of scaffolds for mimicking bone.