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Rong Li
Navy Medical Center of PLA
Corresponding Author
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Background
Multiple myeloma (MM) remains an incurable hematologic malignancy mainly due to its cytogenetic abnormalities. It is important to establish permanent malignant MM cell lines as effective tools to develop more effective therapies.
Results
We established and characterized a new multiple myeloma (MM) cell line CZ2 from the pleural effusion of a 70-year-old man. Using nephelometry and flow cytometry, cells with typical plasma cell morphology but not classical plasma cell phenotype were found to be non-immunoglobulin-secretary cells. FISH analysis of cells revealed a unique characteristic that there is only gain of 1q21 region(1q21+), while no other common cytogenetic abnormalities of multiple myeloma such as deletion of 17p(17p-), deletion of 13q(13q-) and translocation of IgH. In addition, the original cell line maintains its single cytogenetic abnormality. Meanwhile, CKS1B, an adverse prognostic gene which is located in 1q21 region was highly expressed in CZ2 using western blotting. Knockdown of CKS1B could reduce cell viability in addition that cleaved PARP and cleaved caspase3 would be decreased.
Conclusions
Therefore, CZ2 provides a suitable material for cellular and molecular studies of multiple myeloma with only 1q21 abnormality. The cell line characterized by gain of 1q21 and high expression of CKS1B is an important model for studies of myeloma cell growth and drug resistance during therapy.
Keywords
multiple myeloma, cell line, 1q21 gain/amplification, CKS1B
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A new preprint design is coming!
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research
Rong Li
Navy Medical Center of PLA
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 26 Oct, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Molecular Genetics
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Multiple myeloma (MM) remains an incurable hematologic malignancy mainly due to its cytogenetic abnormalities. It is important to establish permanent malignant MM cell lines as effective tools to develop more effective therapies.
Results
We established and characterized a new multiple myeloma (MM) cell line CZ2 from the pleural effusion of a 70-year-old man. Using nephelometry and flow cytometry, cells with typical plasma cell morphology but not classical plasma cell phenotype were found to be non-immunoglobulin-secretary cells. FISH analysis of cells revealed a unique characteristic that there is only gain of 1q21 region(1q21+), while no other common cytogenetic abnormalities of multiple myeloma such as deletion of 17p(17p-), deletion of 13q(13q-) and translocation of IgH. In addition, the original cell line maintains its single cytogenetic abnormality. Meanwhile, CKS1B, an adverse prognostic gene which is located in 1q21 region was highly expressed in CZ2 using western blotting. Knockdown of CKS1B could reduce cell viability in addition that cleaved PARP and cleaved caspase3 would be decreased.
Conclusions
Therefore, CZ2 provides a suitable material for cellular and molecular studies of multiple myeloma with only 1q21 abnormality. The cell line characterized by gain of 1q21 and high expression of CKS1B is an important model for studies of myeloma cell growth and drug resistance during therapy.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
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