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Research article
Rong Zhang
Corresponding Author
ORCiD: https://orcid.org/0000-0003-0092-0889
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Background: Studies of microbiota composition of infants with small intestinal ostomy due to various etiologies are limited. Here, we characterized the intestinal microbiota of neonates with ileostomy resulting from distinct primary diseases.
Methods: Fifteen patients with necrotizing enterocolitis, eight patients with meconium peritonitis, and seven patients with Hirschsprung's disease were included in the study. The small intestinal microbiota composition in infants with ileostomy caused by these diseases was studied.
Results: Microbial diversity in neonatal ileostomy fluid was generally low, and was dominated by members of the Proteobacteria and Firmicutes phyla. At the genus level, the most abundant were Klebsiella, Escherichia-Shigella, Streptococcus, Clostridium sensu stricto 1, Enterococcus, and Lactobacillus. Streptococcus and Veillonella are related to carbohydrate metabolism and immunity, and breastfeeding can increase the proportion of these potentially beneficial bacteria. The proportion of Bifidobacterium in the breastfeeding group was higher than that in the non-breastfeeding group, and incidence of colitis and sepsis was reduced in the breastfeeding group. The proportion of Bifidobacterium increased and incidence of colitis and sepsis decreased in the breastfeeding group compared with the non- breastfeeding group, but there was no significant difference. The increase in body weight in the breastfeeding group was observed to be higher than in the non-breastfeeding group.
Conclusions: Excessive Klebsiella and Escherichia-Shigella and low abundance of Streptococcus, Veillonella and Faecalibacterium suggests that the small intestinal microbiota is in an unhealthy state after ileostomy. However, Streptococcus, Faecalibacterium, and Veillonella species were frequently present, suggesting that expansion of these bacteria might assist the development of the immune system after surgery.
Keywords
Ileostomy, Microbiota, Necrotizing enterocolitis, Nutrition, Infant
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CURRENT STATUS: Published
View the published article at BMC Gastroenterology.
Version 4
Posted 15 Jun, 2020
No community comments so far
Editorial decision: Accept
On 02 Jul, 2020
Review #2 received
Received 30 Jun, 2020
Reviewer #2 agreed
On 29 Jun, 2020
Review #1 received
Received 24 Jun, 2020
Reviewers invited
Invitations sent on 24 Jun, 2020
Reviewer #1 agreed
On 24 Jun, 2020
Editor assigned
On 11 Jun, 2020
Submission checks complete
On 10 Jun, 2020
Editor invited
On 10 Jun, 2020
No comments provided
Review #2 received
Received 03 Jun, 2020
Editorial decision: Major revision
On 03 Jun, 2020
Reviewer #2 agreed
On 20 May, 2020
Review #1 received
Received 14 Apr, 2020
Reviewers invited
Invitations sent on 22 Mar, 2020
Reviewer #1 agreed
On 22 Mar, 2020
Editor assigned
On 12 Mar, 2020
Submission checks complete
On 11 Mar, 2020
Editor invited
On 11 Mar, 2020
No comments provided
Editorial decision: Major revision
On 04 Mar, 2020
Review #2 received
Received 03 Mar, 2020
Review #1 received
Received 21 Feb, 2020
Reviewer #1 agreed
On 13 Feb, 2020
Reviewer #2 agreed
On 13 Feb, 2020
Reviewers invited
Invitations sent on 12 Feb, 2020
Editor assigned
On 07 Feb, 2020
Submission checks complete
On 06 Feb, 2020
Editor invited
On 06 Feb, 2020
No comments provided
Editorial decision: Major revision
On 13 Jan, 2020
Review #2 received
Received 10 Jan, 2020
Review #1 received
Received 05 Jan, 2020
Reviewer #2 agreed
On 23 Dec, 2019
Reviewer #1 agreed
On 21 Dec, 2019
Editor assigned
On 20 Dec, 2019
Reviewers invited
Invitations sent on 20 Dec, 2019
Submission checks complete
On 11 Dec, 2019
Editor invited
On 09 Dec, 2019
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A new preprint design is coming!
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See the published version of this preprint at BMC Gastroenterology.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Rong Zhang
Corresponding Author
ORCiD: https://orcid.org/0000-0003-0092-0889
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Gastroenterology.
Version 4
Posted 15 Jun, 2020
No community comments so far
Editorial decision: Accept
On 02 Jul, 2020
Review #2 received
Received 30 Jun, 2020
Reviewer #2 agreed
On 29 Jun, 2020
Review #1 received
Received 24 Jun, 2020
Reviewers invited
Invitations sent on 24 Jun, 2020
Reviewer #1 agreed
On 24 Jun, 2020
Editor assigned
On 11 Jun, 2020
Submission checks complete
On 10 Jun, 2020
Editor invited
On 10 Jun, 2020
No comments provided
Review #2 received
Received 03 Jun, 2020
Editorial decision: Major revision
On 03 Jun, 2020
Reviewer #2 agreed
On 20 May, 2020
Review #1 received
Received 14 Apr, 2020
Reviewers invited
Invitations sent on 22 Mar, 2020
Reviewer #1 agreed
On 22 Mar, 2020
Editor assigned
On 12 Mar, 2020
Submission checks complete
On 11 Mar, 2020
Editor invited
On 11 Mar, 2020
No comments provided
Editorial decision: Major revision
On 04 Mar, 2020
Review #2 received
Received 03 Mar, 2020
Review #1 received
Received 21 Feb, 2020
Reviewer #1 agreed
On 13 Feb, 2020
Reviewer #2 agreed
On 13 Feb, 2020
Reviewers invited
Invitations sent on 12 Feb, 2020
Editor assigned
On 07 Feb, 2020
Submission checks complete
On 06 Feb, 2020
Editor invited
On 06 Feb, 2020
No comments provided
Editorial decision: Major revision
On 13 Jan, 2020
Review #2 received
Received 10 Jan, 2020
Review #1 received
Received 05 Jan, 2020
Reviewer #2 agreed
On 23 Dec, 2019
Reviewer #1 agreed
On 21 Dec, 2019
Editor assigned
On 20 Dec, 2019
Reviewers invited
Invitations sent on 20 Dec, 2019
Submission checks complete
On 11 Dec, 2019
Editor invited
On 09 Dec, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Gastroenterology.
Background: Studies of microbiota composition of infants with small intestinal ostomy due to various etiologies are limited. Here, we characterized the intestinal microbiota of neonates with ileostomy resulting from distinct primary diseases.
Methods: Fifteen patients with necrotizing enterocolitis, eight patients with meconium peritonitis, and seven patients with Hirschsprung's disease were included in the study. The small intestinal microbiota composition in infants with ileostomy caused by these diseases was studied.
Results: Microbial diversity in neonatal ileostomy fluid was generally low, and was dominated by members of the Proteobacteria and Firmicutes phyla. At the genus level, the most abundant were Klebsiella, Escherichia-Shigella, Streptococcus, Clostridium sensu stricto 1, Enterococcus, and Lactobacillus. Streptococcus and Veillonella are related to carbohydrate metabolism and immunity, and breastfeeding can increase the proportion of these potentially beneficial bacteria. The proportion of Bifidobacterium in the breastfeeding group was higher than that in the non-breastfeeding group, and incidence of colitis and sepsis was reduced in the breastfeeding group. The proportion of Bifidobacterium increased and incidence of colitis and sepsis decreased in the breastfeeding group compared with the non- breastfeeding group, but there was no significant difference. The increase in body weight in the breastfeeding group was observed to be higher than in the non-breastfeeding group.
Conclusions: Excessive Klebsiella and Escherichia-Shigella and low abundance of Streptococcus, Veillonella and Faecalibacterium suggests that the small intestinal microbiota is in an unhealthy state after ileostomy. However, Streptococcus, Faecalibacterium, and Veillonella species were frequently present, suggesting that expansion of these bacteria might assist the development of the immune system after surgery.
Figure 1
Figure 2
Figure 3
This is a list of supplementary files associated with this preprint. Click to download.
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