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Background
Lung cancer is the most commonly occurring malignant tumor. Identification of novel diagnostic and prognostic biomarkers for lung cancer is a key research imperative. The role of CENPK in cancer is an emerging area of research. However, the role of CENPK in the progression of lung adenocarcinoma (LAC) is not well characterized.
Methods
In this study, we identified centromere protein K (CENPK) as a potential new gene for lung cancer based on bioinformatics analysis. In addition, in vitro experiments, including were performed to verify the function of this gene. We investigated the expression of CENPK in LAC by bioinformatics analyses of datasets from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases, differential expression analyses, gene ontology (GO) enrichment, kyoto encyclopedia of genes and genomes (KEGG) analysis, and gene set enrichment analysis (GSEA) were conducted to evaluate its diagnostic and prognostic relevance. In vitro experiments including immunohistochemistry analysis, western blot analysis, CCK8 assay, Transwell assay, flow cytometry, wound healing assay, which were conducted to evaluate the biological behavior and role of CENPK in lung cancer cells.
Results
We demonstrated overexpression of CENPK in LAC; in addition, increased expression of CENPK was associated with clinical progression. Moreover, CENPK was found to be an independent risk factor in patients with LAC. Furthermore, we observed activation of CENPK related signaling pathways in patients with LAC.
Conclusions
In summary, our findings indicate a potential role of CENPK in promoting tumor proliferation, invasion, and metastasis and its application as a novel diagnostic and prognostic biomarker of LAC.
Keywords
lung adenocarcinoma, CENPK, novel biomarkers, prognosis
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View the published article at Cancer Cell International.
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Posted 23 Oct, 2020
No community comments so far
Editorial decision: Major revision
On 31 Oct, 2020
Review #3 received
Received 28 Oct, 2020
Reviewer #5 agreed
On 28 Oct, 2020
Review #5 received
Received 28 Oct, 2020
Review #1 received
Received 24 Oct, 2020
Review #2 received
Received 23 Oct, 2020
Reviewers invited
Invitations sent on 22 Oct, 2020
Reviewer #1 agreed
On 22 Oct, 2020
Reviewer #2 agreed
On 22 Oct, 2020
Reviewer #3 agreed
On 22 Oct, 2020
Reviewer #4 agreed
On 22 Oct, 2020
Editor invited
On 18 Oct, 2020
Editor assigned
On 15 Oct, 2020
Submission checks complete
On 14 Oct, 2020
First submitted
On 13 Oct, 2020
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Subject Areas
Cancer Biology
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A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
See the published version of this preprint at Cancer Cell International.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Primary research
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at Cancer Cell International.
Version 1
Posted 23 Oct, 2020
No community comments so far
Editorial decision: Major revision
On 31 Oct, 2020
Review #3 received
Received 28 Oct, 2020
Reviewer #5 agreed
On 28 Oct, 2020
Review #5 received
Received 28 Oct, 2020
Review #1 received
Received 24 Oct, 2020
Review #2 received
Received 23 Oct, 2020
Reviewers invited
Invitations sent on 22 Oct, 2020
Reviewer #1 agreed
On 22 Oct, 2020
Reviewer #2 agreed
On 22 Oct, 2020
Reviewer #3 agreed
On 22 Oct, 2020
Reviewer #4 agreed
On 22 Oct, 2020
Editor invited
On 18 Oct, 2020
Editor assigned
On 15 Oct, 2020
Submission checks complete
On 14 Oct, 2020
First submitted
On 13 Oct, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Cancer Biology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Cancer Cell International.
Background
Lung cancer is the most commonly occurring malignant tumor. Identification of novel diagnostic and prognostic biomarkers for lung cancer is a key research imperative. The role of CENPK in cancer is an emerging area of research. However, the role of CENPK in the progression of lung adenocarcinoma (LAC) is not well characterized.
Methods
In this study, we identified centromere protein K (CENPK) as a potential new gene for lung cancer based on bioinformatics analysis. In addition, in vitro experiments, including were performed to verify the function of this gene. We investigated the expression of CENPK in LAC by bioinformatics analyses of datasets from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases, differential expression analyses, gene ontology (GO) enrichment, kyoto encyclopedia of genes and genomes (KEGG) analysis, and gene set enrichment analysis (GSEA) were conducted to evaluate its diagnostic and prognostic relevance. In vitro experiments including immunohistochemistry analysis, western blot analysis, CCK8 assay, Transwell assay, flow cytometry, wound healing assay, which were conducted to evaluate the biological behavior and role of CENPK in lung cancer cells.
Results
We demonstrated overexpression of CENPK in LAC; in addition, increased expression of CENPK was associated with clinical progression. Moreover, CENPK was found to be an independent risk factor in patients with LAC. Furthermore, we observed activation of CENPK related signaling pathways in patients with LAC.
Conclusions
In summary, our findings indicate a potential role of CENPK in promoting tumor proliferation, invasion, and metastasis and its application as a novel diagnostic and prognostic biomarker of LAC.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10
This is a list of supplementary files associated with this preprint. Click to download.
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