Cytokeratin-positive cells in the bone marrow from patients with pancreatic, periampullary malignancy and benign pancreatic disease show no prognostic information

doi:10.21203/rs.2.18905/v3

Background: Pancreatic and periampullary carcinoma are aggressive tumours where preoperative assessment is challenging. Disseminated tumour cells (DTC) in the bone marrow (BM) are associated with impaired prognosis in a variety of epithelial cancers. In a cohort of patients with presumed resectable pancreatic and periampullary carcinoma, we evaluated the frequency and the potential prognostic impact of the preoperative presence of DTC, defined as cytokeratin-positive cells detected by immunocytochemistry (ICC).

Methods: Preoperative BMsamples from 242 patients selected for surgical resection of presumed resectable pancreatic and periampullary carcinoma from 09/2009 to 12/2014, were analysed for presence of CK-positive cells by ICC. The median observation time was 21.5 months. Overall survival (OS) and disease-free survival (DFS) were calculated by Kaplan-Meier and Cox regression analysis.

Results: Successful resections of malignant tumours were performed in 179 of the cases, 30 patients resected had benign pancreatic disease based on postoperative histology, and 33 were deemed inoperable intraoperatively due to advanced disease. Overall survival for patients with resected carcinoma was 21.1 months (95% CI: 18.0-24.1), for those with benign disease OS was 101 months (95% CI: 69.4-132) and for those with advanced disease OS was 8.8 months (95% CI: 4.3-13.3).The frequency of CK-positive cells was 6/168 (3.6%) in resected malignant cases, 2/31 (6.5%) in advanced disease and 4/29 (13.8%) in benign disease. The presence of CK-positive cells was not correlated to OS or DFS, neither in the entire cohort nor in the subgroup negative for circulating tumour cells (CTC).

Conclusions: The results indicate that CK-positive cells may be present in both patients with malignant and benign diseases of the pancreas. Detection of CK-positive cells was not associated with differences in prognosis for the entire cohort or any of the subgroups analysed.

Trial registration: clinicaltrials.gov (NCT01919151)

Oncology

Gastroenterology & Hepatology

DTC

disseminated tumour cells

bone marrow

pancreatic cancer

periampullary cancer

According to their anatomical origin, the main carcinoma types of the periampullary region are pancreatic ductal adenocarcinoma (PDAC), distal bile duct cancer (DBDC), ampullary cancer (AMPUC), and duodenal cancer (DUODC). They constitute an entity of aggressive tumours, particularly PDAC and DBDC where 80% of the cases have either locally advanced or metastatic disease at the time of diagnosis (1,2). These tumours are potentially curable when diagnosed at an early, localised stage (3). Still, the results from state of the art “surgery first” strategy (4), with radical resection followed by adjuvant therapy, are dismal. The expected rate of relapse during the first year after surgical resection is 50% and 5-year survival rates below 10% (1,3).

The BM is a known reservoir for dormant or slowly proliferating, disseminated tumour cells (DTCs) (5), being part of the metastatic cascade in malignancies of epithelial origin (6). The best validated DTC-detection method for BM-samples is based on density centrifugation and immunocytochemical (ICC) identification of a panel of Cytokeratins (CK). The morphological evaluation and categorization of the cells follows internationally standardized criteria by the ISHAGE-group (7-9). Beyond prognostic information, there is well established evidence for the clinical utility of DTC detection in the management of the early stages of both breast (10,11) and prostate cancer(5,12). There are several reports on the impact of DTC in pancreatic cancer in general, indicating an association of DTC with reduced survival (13-17). However, for the potential application as a predictive tool for presumed resectable periampullary cancers, the clinical relevance of DTC is unclear. A recent meta-analysis exploring the significance of CTC and DTC in PDAC-patients (18) revealed a significant association between DTC and survival for the entire cohort containing a mix of resectable and advanced cancers. In the subgroup of resected cancers only, the same study did not disclose a significant association, even though another study(13) had reported an association between DTC and impaired survival previously.

The aim of the present study was to explore the frequency and the prognostic impact of preoperatively detected DTCs in a cohort of patients with presumed resectable pancreatic and other periampullary cancers.

Patients, Study design and Follow-up

Between September 2009 and December 2014, patients referred to Oslo University Hospital, Norway with potentially resectable pancreatic and periampullary malignancy were included in a standardised preoperative workup and evaluation in a multidisciplinary tumour board as detailed previously (19). Clinical data were recorded prospectively in an Epi-Info 3.5.3 database (CDC; Atlanta, GA; USA). The follow up included clinical status, computed tomography (CT) of the chest and abdomen as well as CA19-9 assessment twice a year. The mortality was deducted from the Norwegian Cause of Death Registry, provided by the Norwegian Institute of Public Health. The same patient cohort was described previously (20), with the observation period further extended by 25 months to January, 31^st 2019. The study was undertaken in accordance to the STROBE(2014) and REMARK(2012) criteria for analysis and reporting.

Detection of CK-positive cells in the bone marrow

BM samples were collected under anaesthesia just prior to surgery, five ml BM from the anterior iliac crest bilaterally in syringes containing 200IE Heparin in 0.5ml NaCl. Samples were processed within 24h at the Micrometastasis Laboratory, Oslo University Hospital, Norway as previously described (21). BM mononuclear cells were isolated by density centrifugation over a Ficoll-Hypaque gradient (Lymphoprep^®, STEMCELL Technologies UK Ltd., Cambridge, UK) and cytospin-slides were prepared with 0.5x10⁶ BM-MNC/slide. For each sample 4 slides ( i.e. 2x10⁶ cells) were immunostained for CK-positive cells with a combination of the monoclonal mouse primary antibodies AE1 and AE3 (Prod.# MAB 1612 & 1611, Milipore), with a broad affinity for the acidic and basic cytokeratin types, namely CK 1-8, 10, 14-16 and 19. The APAAP method for detection used a rabbit anti-mouse secondary antibody (Dako, #Z0259) and an alkaline phosphatase-mouse-anti-alkaline phosphatase tertiary antibody(Dako, #D0651), followed by a colour reaction with New Fuchsin, staining positive cells red (22). Nuclear counterstaining was performed with haematoxylin. The cytospins were screened for ICC-positive cells in an automated Ariol SL50 analyser (Leica biosystems). Detected elements were reviewed by a trained research engineer (CBS) and candidate cells were classified by a dedicated pathologist (EB). The cytomorphological evaluation of detected ICC-positive cells was performed in accordance to the ISHAGE consensus guidelines(7,8).This protocol, originally validated for breast cancer samples, has become the de facto standard for DTC reporting, including pancreatic cancer studies(13). Based on this classification, and according to earlier practice(23), the ICC-positive cells are divided into 4 categories: tumour cells (TC), hematopoietic (ie “false positive”) cells (HC), QHC (questionable/probable HC) and uninterpretable cells (UIC). If cells classified as TC or UIC were detected, 4 additional cytospins were incubated with the non-reactive mouse monoclonal antibody MOPC21 (Prod# M9269, Sigma Aldrich) of the same Ig isotype as AE1AE3, and detected by APAAP as above, constituting a negative control for the ICC reaction. Samples harbouring cells classified as TC in AE1/AE3 slides and not in the corresponding negative control slides were classified as “DTC-positive”. Samples harbouring cells classified as TC in AE1/AE3 slides and in the corresponding negative control slides were interpreted as “not evaluable” (n.e.) and excluded from further analysis. Samples harbouring “UIC”, “HC” or “QHC” were interpreted as “DTC-negative”. Results were stored in a database at the Micrometastasis Laboratory, Dept. of Pathology, Oslo University Hospital and were not available to treating clinicians. Following closure of the observation period, the classification of ICC-positive cells was combined with the clinical data using a scripted tool upon import to SPSS.

Characteristics of the patient cohort

Patients were categorised into three distinct clinical groups. Patients who underwent surgical resection did either have confirmed malignancy (resectable cancer) or a non-malignant condition (benign disease). The third group consisted of advanced cancer patients who underwent exploratory laparotomy but did not undergo resection, either due to locally advanced tumour growth or overt metastases (advanced cancer).

In addition to the presence of ICC-positive cells, the following clinical- and pathological parameters were recorded: age, gender, CA19-9, tumour size on CT-scan, AJCC/UICC-stage (7^th ed.), pTNM-staging including resection margin, cancer origin, grade of histopathological differentiation, histological subtype (predominantly intestinal or pancreatobiliary), vascular and perineural infiltration. Continuous variables were dichotomized at the following thresholds: CA19-9 ≥ 200kU/L and the size of the lesion on CT-scan ≥ 25mm (for results, see Table 1).

Statistics

Data were analysed in IBM SPSS, V25 (IBM Cooperation Analytics, Armonk, NY, USA) and STATA 15 (Stata Corp LLC, College Station, Texas, USA). Graphs were prepared in PRISM 8 (GraphPad Software Inc., La Jolla, CA, USA). The primary endpoints of the study were overall survival (OS), defined as survival until death by any cause and disease-free survival (DFS), defined as survival until signs of local relapse or metastasis were detected. Survival analyses were carried out with the Kaplan-Meier method, using the Log-rank test for difference of curve pairs. The association between TC-status and survival was quantified by a hazard ratio (HR) with a 95% confidence interval (CI) using Cox regression analysis. Statistical significance was assumed for p<0.05.

Patient group characteristics

277 patients were assessed during the study period of whom 35 patients (12.6%) did not meet the inclusion criteria. Tumour resection with curative intent was performed in 86.4% of the patients (209/242), while in 13.6% (33/242) resection was not performed due to intraoperative detection of advanced disease. In patients deemed unresectable intraoperatively, a biliary or intestinal bypass procedure was performed in 24 patients and 9 received an explorative laparotomy. Of the cases with advanced disease, the tumour origin was the pancreatic head in 30 cases, the duodenum in two cases and in one case the distal bile duct. Of the 209 cases where a successful resection was performed, malignant tumours were confirmed in 85.6% (179/209), while 14.4% (30/209) a benign pancreatic disease was found. Thus, for the whole cohort, resected malignancies account for 74.0% (179/242), advanced disease 13.6% (33/242) and benign pancreatic disease for 12.4% (30/242). The 90-day mortality in operated patients was 2.1% (5/242), all due to rapid cancer progression because of advanced disease, in one case additionally complicated by an anastomotic failure. 84.6% (31/212) of all patients with malignant disease were alive at the date of last follow-up. Ten patients had died from other causes than cancer relapse. Further details on clinical features, distribution of procedures and histopathological diagnoses of the cohort are summarised in Table 1.

Overall survival for patient groups

The median observation time for the entire cohort was 21.5 months (range 1.5-110). Median overall survival (OS) was estimated to 101 months (95% CI: 69.4-132) in patients with a benign lesion, 21.1 months (95% CI: 18.0-24.1) in the resectable cancer group and 8.8 months (95% CI: 4.3-13.3) in patients with advanced cancer. Differences in OS between the subgroups were statistically significant (see also Figure 2).

Bone marrow ICC-positive cell types and patient survival

ICC-positive cells of the named subtypes were identified both in patients with resected malignant tumours, in those with advanced cancers as well as in patients with benign pancreatic disease. According to patient group, DTCs were present in 6/168 (3.6%) in resected cancers, 2/31 (6.5%) in advanced cancers and 4/29 (13.8%) in cases of benign pancreatic disease. The frequency of the other classes of ICC-positive cells in each prognostic group is presented in detail in Table 1. A per case specification of the distribution and frequency of all cell types together with the clinicopathological data are presented in Appendix Table 1. The low number of ICC-positive samples precludes subgroup analysis by tumour type. Survival analyses according to presence or absence of DTCs and the other ICC-positive cells are presented in Figure 3 and Figure 4, and in further detail in Table 2. DTC-positivity was associated with neither OS nor DFS. Furthermore, presence of other ICC-positive cells did not negatively impact on survival. The association between survival and presence of ICC-positive cells for benign disease was hampered by too few ICC-positive cases.

To account for the effect of CTC status and nodal status, as well as the weaker effects of vascular infiltration and histological type on survival of resected patients(20), we performed survival analyses subgrouped by these factors(Figure 5). Patients with or without DTCs had similar outcome in those subgroups (Figure 5). Presence other any other of the bone marrow ICC-positive cell types did not affect survival among the CTC-megative patients (Appendix Figure 1). Among the CTC-positive patients, DTCs were not observed (Figure 5) and in only one patient a single ICC-positive cell (HC) was detected (Appendix Figure 1).

DTC frequency

A low frequency of DTCs was detected in both resected (3.6%) and advanced cancer patients (6.5%). These numbers are less than half the frequencies reported from the largest patient series prior to the present study (13) which reported 13.5% DTC-positivity for resected cancers and 13.9% for advanced cancers. Even higher positive rates of 38% to 57.1% were reported from earlier cohorts with smaller patient numbers and predominantly advanced stage cancers (14,15,17). Complementary to the data from ICC-based studies, there were two studies using RT-PCR for CK-20 (24) detecting 33% positive cases in a cohort of resectable PDAC (24) and CK-19 (25), detecting 67% positives in a cohort of mixed stage cases (25). In the absence of comparative studies between ICC and PCR-based detection, the seemingly higher positive rates for PCR-based assays targeting a single CK category versus ICC utilising broad spectrum antibodies against a range of class I and class II CK-subtypes remains unexplained, although different sensitivity and/or specificity may contribute.

Survival prediction

Among resected pancreatic cancer patients, five ICC-based studies (13-17) reported a significant association between DTCs and impaired survival in univariate analysis. In one of these studies(13), prognostic information was also retained in multivariate analysis (HR 2.755, p=0.022), while the four other studies did not test independent predictive value of DTC. There are two additional ICC-based studies on the same subject that did not discover an association between DTC status and survival (26,27). When weighing the evidence from these studies, several methodological differences have to be taken into consideration. The ISHAGE-group (7) established the first consensus on the cytomorphological criteria for the classification of ICC-positive cells in the BMfor breast cancer patients. These criteria were later underscored in consensus reports (8,9) but also used in additional studies (23,28)

Several of the studies mentioned above have been conducted prior to this standardisation or do not disclose their adherence to the ISHAGE criteria (14,15,17). Furthermore, there is a lack of standardisation concerning the choice of CK-detection antibodies, only one group (27) used the same antibody combination as in the present study. While not validated separately for gastrointestinal cancer types, the broad reactivity of the AE1 and AE3 antibody combinations which covers a spectrum of both the basic and acidic categories of CKs makes the assumption reasonable that the assay employed in the present study detects CK-positive cells independent from the tissue-type dependent differences in CK-subtype expression. The comparison of our results with the aforementioned studies may also be affected by the small size of several of the studies and mixed cohorts with predominantly advanced cancer stages (14-17). Moreover, the substantial rate of surgical interventions performed outside established criteria for operability may have introduced a hidden stage migration (13). Concerning the association of UIC-type cells with an inferior OS in patients with benign disease, there are only two UIC-positive cases out of 30 patients, rendering the result inconclusive. Although there seemed to be an association of UIC with an improved DFS for UIC-positive patients in the resected group, no difference in overall survival was observed. Since DFS and OS are tightly linked in these patients the clinical relevance of this finding is questionable.

ICC-positive cases in benign pancreatic disease

In the present study, ICC-positive cells meeting the morphological criteria for DTC were detected in four out of 29 patients with benign pancreatic disease, for one more patient the analysis was inconclusive. The majority of ICC-based studies have excluded patients with benign pancreatic disease a priori from the analysis (13-15,17,26). Therefore, previous evidence on the validity of the results from the present cohort is limited. This observed rate of 13% is far above the established rate of 2-3.5% for false positive events for the ICC-based assay (7,15,27,29). The presence of occult malignancy in those patients as the source for those cells is unlikely since none of the patients were diagnosed with cancer later during the observation period and none had a previous history of malignancy. While the nature of those cells cannot be further determined in the present study, there are independent reports on cells of seemingly epithelial origin in the BM and peripheral blood of PDAC-patients. One ICC-based study reported results from four patients with benign disease, disclosing two ICC-positive cases (16). In their conclusion the authors deem their method unfit for clinical use in PDAC due to the unreliability of the assay. Also one of the PCR-based studies report a rate of 11% in cases with benign pancreatic disease, while reporting only one positive case in 20 healthy controls(30). In addition, there is one report disclosing the presence of epithelial cells in the bloodstream of patients with benign pancreatic disease (31). These cells could potentially enter the BMvia the bloodstream and could thereby be detected in BM-samples as well. Another possibility for the non-malignant origin of epithelial cells could be the shedding of epithelial cells from the pancreatic- and bile ducts or the duodenal wall during interventions, especially in the presence of inflammation (32). Interestingly, all of the four ICC-positive cases in benign pancreatic disease in the present study had undergone invasive procedures preoperatively, either ERCP, endoscopic biopsy or bile duct stenting.

The results from our study of a large cohort of patients with presumed resectable periampullary cancer show a low frequency of CK-positive cases in both resectable cancer, advanced cancer group and non-malignant pancreatic disease. No association with CK-status and survival was observed. For the UIC cell type, an association with disease free-survival was observed, but this was not supported by overall survival data and the relevance of the association is questionable. Cells satisfying predefined consensus criteria for DTC/CK-positive cells in the BM were detected both in patients with malignant as well as benign pancreatic disease. The combination of low frequency of CK-positive cells and their presence in benign pancreatic disease cases indicates that the ICC-based CK-detection method used in the present study does not reliably identify relevant cells of malignant origin for the type of cancers studied. Nevertheless, additional studies including further characterisation of the cells, preferably with single cell techniques are encouraged.

AJCC/UICC: American Joint Committee on Cancer / Union Internationale Contre le Cancere

AMPUC: Ampullary cancer

APAAP: Alkaline Phosphatase-Anti-Alkaline Phosphatase

BM: Bone marrow

CDC: Centre for Disease Control

CI: Confidence interval

CK Cytokeratin

CT Computed tomography X-ray

CTC: Circulating tumour cells

DBDC: Distal bile duct cancer

DFS: Disease-free survival

DTC: Disseminated tumour cells

DUODC: Duodenal cancer

HC: Hematopoietic cells

ICC: Immuno-cytochemistry

ISHAGE: International Society of Hematotherapy and Graft Engineering

MNC: Mononuclear cells

OS: Overall survival

PDAC: Pancreatic ductal adenocarcinoma

pTNM Pathological tumour stage, nodal status, metastastis status

QHC: Questionable/probable hematopoietic cells

REMARK: Reporting recommendations for tumour marker prognostic studies

STROBE: Strengthening the reporting of observational studies in epidemiology

TC: Tumour cells

UIC: Uninterpretable cells

Ethics approval and consent to participate

All patients selected for surgery were offered participation in this prospective, observational study and asked for their written informed consent. Exclusion criteria were a history of other malignancies in the past ten years, failure to adhere to standard treatment and infrequent cancer types such as neuroendocrine carcinoma. The study protocol was approved by the Regional Ethical Committee (Helse Sør-Øst; Ref. 93-08172d 6.2008.540) in Oslo, Norway and registered at clinicaltrials.gov (NCT01919151). Clinical data were recorded prospectively in a database that was approved by and maintained as regulated by the Oslo University Hospital guidelines, supervised by the Data Protection Officer for Research.

Consent for publication

Not applicable

Availability of data and materials

The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request

Competing interests

None of the authors has any conflicting interest to disclose

Funding

This work was supported by a research grant from Helse Sør-Øst (Regional health-authority) to GW that covered the costs of laboratory analyses for the present study. HH had a part-time junior researcher position funded by Helse Sør-Øst over four years in the beginning of the study period. The funding entity was neither involved in the design of the present study nor were results disclosed previous to publication.

Authors' contributions

The concept of the present study was developed by GW, HH, KJL and BN. The acquisition of clinical data was performed by KJL, HH, LTS, the histopathological data by CSV. CTC analyses and the interpretation of results were performed by CBS, AR and EB. Statistical analyses were performed by CB. All authors were involved in the interpretation of the data with full access to the primary data and have approved the submitted version. The authors have agreed to be personally accountable for their own contributions and to ensure that questions related to the integrity and accuracy of any part of the present work are appropriately investigated, resolved and the resolution documented in the literature.

Acknowledgements

Not applicable

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Table 1: Clinical characteristics of the cohort according to patient groups including ICC-positive cell type status in bone marrow

	Resected cancers (n=179)	Advanced cancers (n=33)	Benign (n=30)
Age, median [years]	69 (34-88)	63 (46-83)	69 (45-82)
Sex, male	92 (51.4%)	19 (57.6%)	21 (70.0%)
ICC-positivity: ≥ 1 ICC-positive cell / 2x 10⁶MNC (ICC-status numbers may differ due to varying number of inconclusive ICC-results per category)
TC UIC HC QHC	6/168 (3.6%) 10/174 (5.7%) 16/169 (9.5%) 12/135 (8.8%)	2/31 (6.5%) 0/31 (0.0%) 2/30 (6.7%) 3/28 (7.9%)	4/29 (13.8%) 2/30 (6.7%) 3/29 (10.3%) 3/25 (12.0%).
Preoperative Risk Factors
CA19-9 ≥ 200kU/l	51/127 (40.2%); 52 missing	17/28 (60.7%); 5 missing	0/20; 10 missing
Tumour size> 25mm	71 (39.7%)	20 (60.6%)	10 (33.3%)
Bilirubin > 50µmol/L	132/151 (87.4%); 28 missing	21/32 (65.6%); 1 missing	7/29 (24.1%); 1 missing
Treatment
Neoadjuvant chemotherapy	GEMZ 6 (4.1%) FOLFIRINX 1 (0.7%)	GEMZ 1 (3.0%)	none
Operation: PPPD PD Pancreatectomy	146 (82.6%) 25 (14.0%) 8 (4.5%)	BDB 24 (72.7%) Exp.lap. 9 (27.3%)	29 (96.7%) 1 (3.3%) none
Venous resection	49 (27.4%)	n.a.	2 (6.7%)
Adjuvant chemotherapy	FLV: 91 (50.8%) GEMZ: 7 (3.9%) FLOX: 1 (0.7%) none: 80 (44.7%)	Palliative chemotherapy 31 (93.9%)	n.a.
Histopathologic results
Pancreatic cancer Malignant IPMN Distal bile duct ca. Ampullary cancer Duodenal cancer	101 (56.4%) 9 (5.0%) 31 (17.3%) 21 (11.7%) 17 (9.4%)	30 1 2
Pancreatobiliary type Intestinal type Mucinous type	146 (81.6%) 26 (14.5%) 7 (3.9%)	28 (84.8%) 5 (15.2%)	IPMN 11 (36.7%) ben.Tu. 5 (16.6%) AIP 6 (20.0%) chr.Panc 4 (13.3%) inflam. 4 (13.4%)
UICC-stage (V7): I II III IV	16 (10.6%) 150 (83.8%) 13 (7.3%)	7 (21.2%) 26 (78.8%)
N1-status	120 (67.0%)
R1-status	87 (48.6%)
Vascular infiltration	108 (60.3%)
Perineural infiltration	141 (78.8%)
GEMZ: Gemzitabine, FLV, FLOX, FOLFIRINOX: chemotherapy regimens; PD: pancreatico-duodenectomy, PPPD: pylorus preserving PD, BDB: biliodigestive bypass; Ex.lap.: explorative laparotomy; IPMN: intraductal pancreatic mucinous neoplasia; ben.tu.: benign tumour; AIP: autoimmune pancreatitis; chr. panc.: chronic pancreatitis; inflam.: inflammation n.a.: not applicable; n.d.: not determined. Note: ICC-status numbers may differ due to varying number of inconclusive ICC-results per category.

Table 2: Overall survival according to ICC-positive cell type status in bone marrow

ICC-type

Level

N

Incident
cases

Person months

HR
(95%CI)

p-value

Mean OS
(95%CI)

Overall survival, resected cancers

TC

Positive

6

5

217.5

1.1 (0.4-2.3)

0.865

36.2 (11.3-61.2)

Negative

162

133

5293.6

37.1 (31.6-42.6)

UIC

Positive

10

7

488.1

0.6 (0.3-1.3)

0.197

52.5 (27.2-77.7)

Negative

164

136

5144.0

35. 6 (30.2-40.9)

HC

Positive

16

14

431.6

1.3 (0.7-2.2)

0.386

29.6 (14.3-44.8)

Negative

153

125

5006.5

37.3 (31.6-43.1)

QHC

Positive

12

10

419.0

0.9 (.5-1.8)

0.795

37.1 (18.1-56.0)

Negative

123

102

3817.1

35.5 (29.2-41.8)

Overall survival, advanced cancers

TC

Positive

2

7.9

3.4 (0.7-15.4)

0.114

3.9 (0.8-7.1)s

Negative

29

298.8

10.3 (7.4-13.2)

UIC

Positive

0

-

Negative

31

306.6

9.9 (7.1-12.7)

HC

Positive

2

14.3

1.6 (0.4-7.2)

0.522

7.2 (4.0-10.3)

Negative

28

266.6

9.5 (6.5-12.5)

QHC

Positive

3

16.6

2.1 (0.6-7.2)

0.262

5.5 (1.9-9.2)

Negative

25

248.5

9.9 (6.5-13.4)

Overall survival, benign disease

TC

Positive

4

2

256.1

4.0 (0.7-22.6)

0.114

65.3 (53.0-77.6)

Negative

25

7

1929.4

92.2 (82.7-101.7)

UIC

Positive

2

143.0

5.7 (1.1-25.5)

0.039

71.5 (52.9-90.1)

Negative

28

7

1796.9

92.6 (83.2-101.9)

HC

Positive

3

2

202.3

4.7 (0.9-25.7)

0.075

69.4 (43.1-95.7)

Negative

26

6

1867.3

93.7 (84.3-103.2)

QHC

Positive

3

2

202.3

3.9 (0.7-21.6)

0.114

5.5 (1.9-9.2)

Negative

22

5

1639.1

9.9 (6.5-13.4)

Disease free survival, resected cancers

TC

Positive

6

112.4

1.3 (0.6-3.0)

0.525

18.7 (4.5-33.0)

Negative

162

126

4022.2

32.2 (25.9-38.5)

UIC

Positive

10

5

441.8

0.4 (0.2-1.0)

0.040

62.1 (37.1-87.1)

Negative

164

132

3785.8

29.4 (23.5-35.4)

HC

Positive

16

299.4

1.4 (0.8-2.5)

0.277

22.6 (5.7-39.5)

Negative

153

120

3774.0

31.9 (25.5-38.3)

QHC

Positive

12

10

340.7

1.0 (0.5-1.9)

0.973

30.1 (10. 1-50.8)

Negative

123

96

2825.6

30.8 (23.7-37.8)

Detailed survival analysis for patients with (pos.) or without (neg.) ICC-positive cells in the bone marrow in the indicated morphological cell categories. HR and p-values were computed by Cox-regression analysis. The number of patients analysed may differ from group size for cases with inconclusive ICC-results.

Table 3. Published studies on DTC status in the bone marrow for peri-ampullary adenocarcinomas, grouped by detection method.

Authors	Method/ Markers	Detection limit	DTC def.	Pat. number	Loc. / Adv.	TC-rate	false-pos. rate	Prog.	Comments
Immunocytochemistry
Effenberger 2012⁽¹³⁾	CK A45-B/B3	≥1 / 2x10⁶MNC	TC	n=175	n=71 / n=104	13.7% (24/175)	-	Pos.	29 pat. resected despite advanced cancer stage
Rehders 2012⁽²⁵⁾	CK A45-B/B3	≥1 / 1x10⁶MNC	TC	n=49	n=49 / -	27% (12/49)	-	Neg.	No details on survival analysis disclosed
Roder 1999⁽¹⁷⁾	CK 2; KL1; A45-B7B3	≥1 / 5x10⁵MNC	TC	n=48	n=8 / n=40	52.1% (25/48)	-	Pos.	Association with OS for resected patients
van Heek 2001⁽¹⁶⁾	CK (8,18)	≥1 / 1x10⁷MNC	TC	n=35	n=14 / n=13	34.2% (12/35)	50% (2/4)	Pos.	2/4 benign pat. false positive
Vogel 1999⁽¹⁴⁾	panCK, mucin, CEA, Ca-19–9	≥1 / 1.25x10⁶	TC	n=80	n=11 / n=60	38% (27/71)	6.6% (3/45)	Pos.
Thorban 1996⁽¹⁵⁾	CK A45-B/B3	≥1 / 5x10⁵MNC	TC	n=42	n=24 / n=18	57.1% (24/42)	-	Pos.	Association with metastatic and local relapse
Z`Graggen 2001⁽²⁶⁾	CK AE3/AE1	≥1 / 5x10⁶MNC	TC	n=54	n=3 / n=51	24% (13/54)	4.1% (1/24)	Neg.
Nucleotide based detection
Hoffmann 2007⁽²⁴⁾	rt-PCR / CK-19	-		n=37	n=7 / n=30	67% (25/37)	-	Neg.
Soeth 2005⁽²³⁾	rt-PCR / CK-20	-		n=117	n=172	33.3% (45/135)	11%	Neg.	Survival data only for blood/BM combined. False positive rate 11%
Prog: prognostic impact of DTC-status on survival Loc: Localised cancers; Adv: advanced cancers; MNC: mononuclear cells

Cytokeratin-positive cells in the bone marrow from patients with pancreatic, periampullary malignancy and benign pancreatic disease show no prognostic information

Status:

Journal Publication

Version 3

Abstract

Figures

Background

Methods

Patients, Study design and Follow-up

Detection of CK-positive cells in the bone marrow

Characteristics of the patient cohort

Statistics

Results

Patient group characteristics

Overall survival for patient groups

Bone marrow ICC-positive cell types and patient survival

Discussion

DTC frequency

Survival prediction

ICC-positive cases in benign pancreatic disease

Conclusions

Abbreviations

Declarations

Ethics approval and consent to participate

Consent for publication

Availability of data and materials

Competing interests

Funding

Authors' contributions

Acknowledgements

References

Tables

Supplementary Files

Status:

Journal Publication

Version 3