Biomass conversion to bioenergy has always been necessary to ensure the most efficient use of the limited biomass resource and enable economic viability. Evaluating biomass transportation cost, electricity transmission cost and heat transferring cost between power plant location/s and supply/demand points and selection of an optimum power plant capacity is an important issue for a robust supply chain design. In this study, we employed designing optimum biomass to the bioenergy supply chain for agricultural activities using Geographic Information System and Simulated Annealing algorithm to overcome a real-world problem in Bismil District of Diyarbakır/Turkey. Our goal is to define a potential investment location/s on the trigeneration system by comparing the trade-offs between the raw material/end-product transportation costs and facility/s and pipeline installation costs. To determine possible locations for power plants, distance matrices were retrieved from suitable candidate power plant locations and agricultural parcel, settlement and the nearest high voltage electricity line from the Geographic Information System. The results showed that establishing one power plant is feasible. The net present value of a potential investment is almost 260 million Euros and the re-payment period is 1.33 years.