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Research article
Li Xu
Henan Institute of Science and Technology
Corresponding Author
ORCiD: https://orcid.org/0000-0001-8875-4445
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Spodoptera litura is a destructive agricultural pest and has evolved resistance to multiple insecticides, especially pyrethroids. At present, the resistance mechanism to pyrethroids remains unclear.
Results
Four field-collected populations, namely CZ, LF, NJ and JD, were identified to have high resistance to pyrethroids comparing to pyrethroid-susceptible population (GX), with increased resistant ratio ranging from 40.8 to 1764.0-fold. To characterize pyrethroid resistance mechanism, the transcriptomes between pyrethroid-resistant (LF and NJ) and pyrethroid-susceptible (GX) populations were compared by RNA-sequencing. Results showed that multiple differentially expressed genes were enriched in metabolism-related GO terms and KEGG pathways. 35 up-regulated metabolism-related genes were screened to verify by qRT-PCR. Consistent up-regulation of 13 unigenes, including 3 P450s, 4 GSTs, 1 UGT, 4 COEs and 1ABC, were verified in the additional pyrethroid resistant populations CZ and JD. The expression levels of CYP3 and GST3 , which were homologous to CYP321A8 and GST1 , respectively, showed positive correlation with their pyrethroid resistance levels within CZ, LF, NJ and JD populations. While the expression levels of CYP12 , CYP14 , COE4 and ABC5 showed good correlation with their pyrethroid resistance levels in at least three populations. UGT5 had the highest expression level among the tested UGT genes in the four pyrethroid resistant populations.
Conclusion
CYP3 , CYP12 , CYP14 , GST3 , COE4 , UGT5 and ABC5 play important roles in pyrethroid resistance among the four field-collected populations. Our work provides a valuable clue for further study of pyrethroid resistance mechanisms in S. litura .
Keywords
Spodoptera litura, pyrethroids, resistance mechanism, transcriptome, metabolism-related
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A new preprint design is coming!
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research article
Li Xu
Henan Institute of Science and Technology
Corresponding Author
ORCiD: https://orcid.org/0000-0001-8875-4445
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
View the published article at Pesticide Biochemistry and Physiology.
Version 1
Posted 16 Dec, 2019
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Epigenetics & Genomics
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Pesticide Biochemistry and Physiology.
Background
Spodoptera litura is a destructive agricultural pest and has evolved resistance to multiple insecticides, especially pyrethroids. At present, the resistance mechanism to pyrethroids remains unclear.
Results
Four field-collected populations, namely CZ, LF, NJ and JD, were identified to have high resistance to pyrethroids comparing to pyrethroid-susceptible population (GX), with increased resistant ratio ranging from 40.8 to 1764.0-fold. To characterize pyrethroid resistance mechanism, the transcriptomes between pyrethroid-resistant (LF and NJ) and pyrethroid-susceptible (GX) populations were compared by RNA-sequencing. Results showed that multiple differentially expressed genes were enriched in metabolism-related GO terms and KEGG pathways. 35 up-regulated metabolism-related genes were screened to verify by qRT-PCR. Consistent up-regulation of 13 unigenes, including 3 P450s, 4 GSTs, 1 UGT, 4 COEs and 1ABC, were verified in the additional pyrethroid resistant populations CZ and JD. The expression levels of CYP3 and GST3 , which were homologous to CYP321A8 and GST1 , respectively, showed positive correlation with their pyrethroid resistance levels within CZ, LF, NJ and JD populations. While the expression levels of CYP12 , CYP14 , COE4 and ABC5 showed good correlation with their pyrethroid resistance levels in at least three populations. UGT5 had the highest expression level among the tested UGT genes in the four pyrethroid resistant populations.
Conclusion
CYP3 , CYP12 , CYP14 , GST3 , COE4 , UGT5 and ABC5 play important roles in pyrethroid resistance among the four field-collected populations. Our work provides a valuable clue for further study of pyrethroid resistance mechanisms in S. litura .
Figure 1
Figure 2
Figure 3
This is a list of supplementary files associated with this preprint. Click to download.
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