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Research article
Xiaonan Yu
College of Landscape Architecture, Beijing Forestry University, Beijing, 100083, P.R. China;
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: The phenomenon of petaloid stamens in Paeonia lactiflora is an important cause of double flower formation. Although research on stamen development in model plants has progressed, the molecular mechanism of P. lactiflora petaloid stamens is still unclear.
Results: In this study, a comparative transcriptomic analysis was performed on two cultivars of P. lactiflora (‘Fen Yu Nu’ and ‘Lian Tai’) with different stamen developmental patterns. Using transcriptome sequencing, 89,393 unigenes were identified in P. lactiflora. Trend analysis and weighted gene co-expression network analysis (WGCNA) indicated that 18 candidate genes were likely involved in petaloid stamens, including seven MADS-box genes PlAP3, PlDEFA, PlPI2, PlAG-1, PlSEP3, PlSEP1-1, and PlSEP1-2, and 11 transcription factors (TFs) PlTCP2, PlTCP4, PlTCP9, PlbHLH36, PlICE1, PlLBD38, PlNAC083, PlBLH11, PlPDF2, PlGBF1, and PlIIIA. Based on the selected candidate genes, a hypothetical model of gene expression network regulating petaloid stamens is proposed.
Conclusions: Our results provide a collection of candidate genes for the analysis of P. lactiflora petaloid stamens, allowing for in-depth studies of the development pattern of P. lactiflora flower organs, and providing a theoretical basis for related research on petaloid stamens of other herbaceous flowers.
Keywords
Comparative transcriptome, Paeonia lactiflora, Petaloid stamens, Trend analysis, Weighted gene coexpression network
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This is a list of supplementary files associated with this preprint. Click to download.
- Additionalfile9.xlsx
Additional file 9: Gene primer pairs for qRT-PCR
- Additionalfile8.xlsx
Additional file 8: DEGs in Ivory and Brown modules
- Additionalfile7.png
Additional file 7: Filtering of power value for gene network weight analysis
- Additionalfile6.xlsx
Additional file 6: Genes that regulate the development of stamens in Arabidopsis thaliana
- Additionalfile5.xlsx
Additional file 5: DEGs in ‘Fen Yu Nu’ (FYN) vs. ‘Lian Tai’ (LT) combinations
- Additionalfile4.png
Additional file 4: Top 20 of GO and KEGG enrichment in profile 6 of ‘Lian Tai’ (LT) cultivar
- Additionalfile3.xlsx
Additional file 3: DEGs of significant enrichment patterns (profiles 6 and 7) in ‘Lian Tai’ (LT) cultivar
- Additionalfile2.png
Additional file 2: Top 20 of GO and KEGG enrichment in profile 7 of ‘Lian Tai’ (LT) cultivar
- Additionalfile1.xlsx
Additional file 1: Throughput and quality of RNA-seq
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A new preprint design is coming!
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research article
Xiaonan Yu
College of Landscape Architecture, Beijing Forestry University, Beijing, 100083, P.R. China;
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 23 Oct, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: The phenomenon of petaloid stamens in Paeonia lactiflora is an important cause of double flower formation. Although research on stamen development in model plants has progressed, the molecular mechanism of P. lactiflora petaloid stamens is still unclear.
Results: In this study, a comparative transcriptomic analysis was performed on two cultivars of P. lactiflora (‘Fen Yu Nu’ and ‘Lian Tai’) with different stamen developmental patterns. Using transcriptome sequencing, 89,393 unigenes were identified in P. lactiflora. Trend analysis and weighted gene co-expression network analysis (WGCNA) indicated that 18 candidate genes were likely involved in petaloid stamens, including seven MADS-box genes PlAP3, PlDEFA, PlPI2, PlAG-1, PlSEP3, PlSEP1-1, and PlSEP1-2, and 11 transcription factors (TFs) PlTCP2, PlTCP4, PlTCP9, PlbHLH36, PlICE1, PlLBD38, PlNAC083, PlBLH11, PlPDF2, PlGBF1, and PlIIIA. Based on the selected candidate genes, a hypothetical model of gene expression network regulating petaloid stamens is proposed.
Conclusions: Our results provide a collection of candidate genes for the analysis of P. lactiflora petaloid stamens, allowing for in-depth studies of the development pattern of P. lactiflora flower organs, and providing a theoretical basis for related research on petaloid stamens of other herbaceous flowers.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10
Figure 11
Figure 12
This is a list of supplementary files associated with this preprint. Click to download.
- Additionalfile9.xlsx
Additional file 9: Gene primer pairs for qRT-PCR
- Additionalfile8.xlsx
Additional file 8: DEGs in Ivory and Brown modules
- Additionalfile7.png
Additional file 7: Filtering of power value for gene network weight analysis
- Additionalfile6.xlsx
Additional file 6: Genes that regulate the development of stamens in Arabidopsis thaliana
- Additionalfile5.xlsx
Additional file 5: DEGs in ‘Fen Yu Nu’ (FYN) vs. ‘Lian Tai’ (LT) combinations
- Additionalfile4.png
Additional file 4: Top 20 of GO and KEGG enrichment in profile 6 of ‘Lian Tai’ (LT) cultivar
- Additionalfile3.xlsx
Additional file 3: DEGs of significant enrichment patterns (profiles 6 and 7) in ‘Lian Tai’ (LT) cultivar
- Additionalfile2.png
Additional file 2: Top 20 of GO and KEGG enrichment in profile 7 of ‘Lian Tai’ (LT) cultivar
- Additionalfile1.xlsx
Additional file 1: Throughput and quality of RNA-seq
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