3.1 Effect of magnets arrangements on water parameters
The water flowed under non-reversed polarity magnets arrangement at flow rate of 0.1–0.5 mL s-1 showed an overall better improvement of pH, ORP and DO concentration in comparison to the flow under reversed polarity magnets arrangement. The percentage changes of pH, ORP and DO concentration for this arrangement were +3.5, -4.2 and +9.5%, respectively (Fig. 3; Table S1). On the contrary, the reversed polarity magnets arrangement yielded smaller percentage changes, which were +2.5, -3.0 and +5.0%, respectively (Fig. 3; Table S1). The magnetic effect generated from non-reversed magnetic poles was almost uniform, as compared to its reversed counterpart which generated a highly discontinuous field [22, 23]. Furthermore, magnets with reversed and non-reversed configurations also corresponded to different magnetic field waveforms, with the latter having more homogenous waveform [24]. Nonetheless, Alabi et al. [25] claimed that the purpose of using any magnetic configuration was to generate a uniform magnetic field within the passage of water flow. Hence, most essentially, the orientation of magnets should be in such a way that the water flow is always perpendicular (90°) to the magnetic field lines [26].
3.2 Effect of flow rate on water parameters
The results in Fig. 4 and Table S2 revealed that when water flowed through magnetic effect at the lowest flow rate, which was 0.1–0.5 mL s-1, a better improvement of pH, ORP and DO concentration was achieved. In this case, the percentage changes of pH, ORP, and DO concentration after magnetization amounted to +3.5, -6.9 and +5.6%, respectively. When flow rate increased, the percentage changes of water parameters decreased. As shown in Fig. 4, when flow rate increased further to 1.0–1.5 mL s-1, the percentage changes decreased to +2.6, -1.8 and +4.8%, respectively. Subsequently, when the highest flow rate (2.0–2.5 mL s-1) was reached, the percentage changes recorded the lowest, which amounted to +0.92, -1.3 and +2.9%, respectively. Similar findings were reported in the study conducted by Kadhim and Al-Rufaye [27] where the average change in water parameters such as pH, ORP and electrical conductivity of well water decreased from +1, +16 and +3% to 0, +1 and +1%, respectively when flow rate increased from 0.1 to 1 L s-1. Besides, the magnetization effect was reported to increase at low flow rate, indicating a longer exposure time of water to magnetic effect [28]. Therefore, these results can show that lower flow rate increases the exposure time of water to magnetic effect, leading to the overall improvement of water parameters.
3.3 Correlation of pH and ORP calculations
Typically, from the experimental findings, when water flowed through magnetic effect, its pH and DO concentration increased whereas its ORP decreased. This trend could be explained through reversed electric motor principle. Primarily, this principle is referenced from the electric motor theory [29] such that when current passes through a conductor coil in a magnetic field, a torque (mechanical force) will be generated which in turn moves the coil. As a result of current passing through the conductor coil, the electron will flow, hence producing electricity. The reversed electric motor principle is precisely the reversed process of electric motor theory, which can also be known as the electric generator principle [30]. In the context of water magnetization, the principle imparts the following attributes to the parameters as represented in Eqs. (4) and (5), respectively [31].
![](https://myfiles.space/user_files/58653_1b1c6aeb34a62c68/58653_custom_files/img1612435699.jpg)
Water molecules ionize to form H+ and OH- ions under equilibrium condition based on Eq. (4).
- Current is induced when magnetic effect is disrupted by the mechanical motion of water flow, which is flowing in the direction perpendicular to the magnetic field.
- The induced current will promote flow of electron.
- The generated electron has the tendency to bind with H+ cation from ionized water molecule through metastable bonding.
- Consequently, pH of water increases and the concentration of OH- anion also increases simultaneously.
- When there is excess OH- anion due to pH increment, the chemical equilibrium is disturbed. As a result, subsequent oxidation of OH- anion occurs to form O2, H2O and electron (Eq. (5)) in order to restore chemical equilibrium based on Le Chatelier’s principle [32, 33].
The presence of electron in water was indicated by the ORP measurement. The principle of ORP measurement states that when ORP value of water decreases, it signifies the increase of electron density in water. This study highlights the significance of pH and ORP due to the reversed electric motor effect. A correlation between pH and ORP can be determined by using the Nernst equation [34] as shown in Eq. (6).
![](https://myfiles.space/user_files/58653_1b1c6aeb34a62c68/58653_custom_files/img1612435731.jpg)
where E, E°, R, T, n, F and [H+] represent the measured potential (ORP value) in V, the standard potential value in V, gas constant (8.31) in J mol-1 K-1, absolute temperature in K, stoichiometric number of exchanged electrons in the reaction, Faraday’s constant (96490) in C mol-1 and hydrogen ions concentration, respectively. The redox activity, in terms of E value can be measured by the ORP sensor using the analogy of electrochemical cell when a potential difference (electron transfer) occurs between the inert or indicating electrode (usually platinum) and the silver/silver chloride (Ag/AgCl) reference electrode. In this study, two possible half reactions of redox reactions can be assumed, which are the reduction of AgCl [35] and oxidation of OH- [36]. They are shown in Eqs. (7) and (8), respectively.
![](https://myfiles.space/user_files/58653_1b1c6aeb34a62c68/58653_custom_files/img1612435765.jpg)
Based on electrochemistry theory and the standard electrode potential values [35, 36], the reduction of AgCl in the ORP sensor represents the cathode with E° = 0.222 V, whereas the oxidation of OH- from water represents the anode with E° = -0.401 V. Thus, the overall E° of the cell (E° cell) is 0.623 V, which is calculated based on Eq. (9) [37].
![](https://myfiles.space/user_files/58653_1b1c6aeb34a62c68/58653_custom_files/img1612435811.jpg)
By using E° = 0.623 V, the experimental and theoretical E values can be calculated based on the measured pH values of water in this study using Eq. (6). By assumption, for every pH increment by 1.00, E value or ORP value would decrease by 0.015 V (Table S3; Fig. S1).
3.4 Electron generation in water due to magnetic effect
From the pH and ORP correlation calculation, it is also possible to estimate the number of electrons generated in water. The estimated number of electrons generated in the control and magnetization experiments based on pH and ORP measurements were calculated as shown in Table S4, and the results summarized in Table 2. The obtained results are based on the data from optimized magnetization variables, which is non-reversed polarity magnets arrangement combined with the lowest flow rate (0.1–0.5 mL s-1).
Table 2 reveals that once water flowed through magnetic effect, pH and ORP values are changed accordingly. The number of electrons could increase from 1.6 × 1014 to 2.4 × 1014, as compared to control which was from 1.5 × 1014 to 1.7 × 1014 when there was no magnetic effect. Meanwhile, these data imply that both pH increment and ORP decrement are associated with the number of electrons generated in water. Subsequently, when water is magnetized, the number of electron generation could amount to 8.0 × 1013, as compared to the control which could amount to only 2.0 × 1013. Therefore, this evidences that pH increment can lead to remarkable increase in electron density and DO concentration in water due to magnetic effect.
3.5 Raman spectral analysis
Figure 5a shows the Raman spectra for unmagnetized and magnetized water in the wavenumber of 2600–4000 cm-1. Both the symmetric and asymmetric OH stretching vibrations of water are located between 2900–3700 cm-1.
Li et al. [38] stated that the typical Raman spectra of water consists of a peak around 3400 cm-1, an intense shoulder around 3200 cm-1 and a weak shoulder near 3600 cm-1. These typical characteristics are observed in the Raman spectra. As seen in Fig. 5a, magnetized water has an overall greater intensities of the Raman peak and the shoulders than that of unmagnetized water, which aligns with the findings of Pang and Deng [39]. This phenomenon was associated to the alteration in water molecular structures and clusters, as well as the transition of valence, bonded and inner-layer electrons due to magnetic effect [39]. The higher electron density in water generated a higher Raman intensity due to higher electronic transition upon excitation by the electromagnetic irradiation (Raman laser) on water [40].
In addition, a higher Raman OH stretching vibration intensity of water could be associated with stronger intermolecular hydrogen bonds of water [41]. This aligns with the findings of Toledo et al. [19] whereby magnetic effect weakens intra cluster hydrogen bonds of water, which promotes smaller water clusters formation with strong inter cluster hydrogen bonds. Based on the finding of Chen et al. [12], electron played a significant role in the transformation of water clusters and alteration of hydrogen bonds. In particular, water with reduced hydrogen bonds via electron-induced mechanism had a greater peak area of Raman spectra compared to normal DI water. Therefore, the finding in this study, which showed that magnetized water had a greater Raman peak area compared to unmagnetized water (Fig. 5b), aligned with literature findings in terms of generating water with increased electron density, reduced hydrogen bonding and smaller cluster size.
3.6 Implications of study
The electron density and DO concentration increment in water can suggest potential applications in essential water-related industries, particularly in disease control. For examples, most recent literatures have highlighted that free radical damage could have contributed to novel Coronavirus SARS-CoV-2 infections that cause the severe Coronavirus pandemic alongside other respiratory infection such as the severe acute respiratory syndrome [42]. Besides, cancer can also be attributed to free radical damage induced by reactive oxygen species [43]. These free radical species in body lack electron which makes them highly reactive. As a result, cells are susceptible to oxidative stress. Higher electron density water may be able to counteract the oxidative stress when free radical species scavenge electron from water. In addition, since this study also substantiates DO increment in water due to magnetic effect, it can also be recommended for the treatment of cancers developed from hypoxia (an environment with decreased oxygen level [44], which is conventionally treated with oxygen nanoshuttles in clinical study [45].