This preprint is under consideration at BMC Ophthalmology. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Zhenggao Xie
Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
To investigate the effect of indocyanine green (ICG) on apoptosis, proliferation, migration, invasion and transdifferentiation in cultured human lens epithelial cells line SRA01/04 (HLECs).
Methods
HLECs were exposed to ICG for 3 minutes at various concentrations of 0.5%, 1.5% and 2.5%. The apoptosis process was detected by flow cytometry, and apoptosis related proteins Bax, Bcl-2, caspase-3 and caspase-9 were detected by Western blot. Cell proliferation process was detected via the cell counting kit-8 (CCK-8) assay and 5-ethynyl-2'-deoxyuridine (EdU) incorporation assay. Wound healing assay was employed to determine cell migration. Transwell assays were performed to assess cell migration and invasion process. Transdifferentiation related proteins α-SMA, N-cadherin, Fibronectin and Vimentin were accessed by Western blot.
Results
ICG significantly increased HLECs apoptosis and decreased cell proliferation, migration, invasion and transdifferentiation after 3 minutes of exposure. Expression of Bax, caspase-3, and caspase-9 pro-apoptosis proteins were up-regulated after ICG exposure, while Bcl-2 anti-apoptosis protein was down-regulated. Transdifferentiation related proteins α-SMA, N-cadherin, Fibronectin and Vimentin expression were down-regulated.
Conclusions
ICG can promote HLECs apoptosis and inhibition HLECs proliferation, migration, invasion and transdifferentiation in a concentration-dependent manner.
Keywords
SRA01/04, Indocyanine green, Posterior capsule opacification
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
No competing interests reported.
Loading...
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 20 Oct, 2021
No community comments so far
Reviews received
Received 16 Nov, 2021
Reviewers agreed
On 14 Nov, 2021
Reviews received
Received 09 Nov, 2021
Reviewers agreed
On 04 Nov, 2021
Reviewers invited
Invitations sent on 04 Nov, 2021
Editor assigned
On 04 Nov, 2021
Editor invited
On 18 Oct, 2021
Submission checks complete
On 18 Oct, 2021
First submitted
On 08 Oct, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Ophthalmology
Learn more about our company.
This preprint is under consideration at BMC Ophthalmology. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Zhenggao Xie
Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 20 Oct, 2021
No community comments so far
Reviews received
Received 16 Nov, 2021
Reviewers agreed
On 14 Nov, 2021
Reviews received
Received 09 Nov, 2021
Reviewers agreed
On 04 Nov, 2021
Reviewers invited
Invitations sent on 04 Nov, 2021
Editor assigned
On 04 Nov, 2021
Editor invited
On 18 Oct, 2021
Submission checks complete
On 18 Oct, 2021
First submitted
On 08 Oct, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Ophthalmology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
To investigate the effect of indocyanine green (ICG) on apoptosis, proliferation, migration, invasion and transdifferentiation in cultured human lens epithelial cells line SRA01/04 (HLECs).
Methods
HLECs were exposed to ICG for 3 minutes at various concentrations of 0.5%, 1.5% and 2.5%. The apoptosis process was detected by flow cytometry, and apoptosis related proteins Bax, Bcl-2, caspase-3 and caspase-9 were detected by Western blot. Cell proliferation process was detected via the cell counting kit-8 (CCK-8) assay and 5-ethynyl-2'-deoxyuridine (EdU) incorporation assay. Wound healing assay was employed to determine cell migration. Transwell assays were performed to assess cell migration and invasion process. Transdifferentiation related proteins α-SMA, N-cadherin, Fibronectin and Vimentin were accessed by Western blot.
Results
ICG significantly increased HLECs apoptosis and decreased cell proliferation, migration, invasion and transdifferentiation after 3 minutes of exposure. Expression of Bax, caspase-3, and caspase-9 pro-apoptosis proteins were up-regulated after ICG exposure, while Bcl-2 anti-apoptosis protein was down-regulated. Transdifferentiation related proteins α-SMA, N-cadherin, Fibronectin and Vimentin expression were down-regulated.
Conclusions
ICG can promote HLECs apoptosis and inhibition HLECs proliferation, migration, invasion and transdifferentiation in a concentration-dependent manner.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
No competing interests reported.
Loading...