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Research article
Xinglong Wang
Northwest Agriculture and Forestry University
Corresponding Author
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Background Myostatin (MSTN) and somatostatin (SST) are all involved in regulation the growth of the animals. Animals immunized with these proteins induce the related immune response, which could neutralize the normal inhibitory effects of these two proteins. Here, a recombinant adenovirus expressing MSTN-SST was constructed and used to deliver these two antigen to mice, so as to check the immunize efficacy of these proteins delivered in this way. Results The adenovirus expression fusing protein MSTN-SST was successfully rescued on HEK293A cell as adenovirus related cell pathological change were observed after transfection with the adenovirus genome harboring MSTN-SST gene. The expression of MSTN-SST were confirmed using western blotting and indirect immunofluorescence. Immunization with the recombinant adenovirus once or twice were successfully induce immune response against MSTN-SST and resulted in an improved growth rate and muscle mass. In addition, a booster immunization leaded a better results shown as higher antibody response and mice growth rate. Conclusions Accordingly, adenovirus can be used as a vector for deliver myostatin and somatostatin gene to increase animal growth rate and muscle mass .
Keywords
myostatin, somatostatin, growth rate, muscle mass
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research article
Xinglong Wang
Northwest Agriculture and Forestry University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
The most recent version of this article is available here.
No community comments so far
No comments provided
No comments provided
No comments provided
Version 2
Posted 09 Jan, 2020
No comments provided
No comments provided
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Small Animal Medicine
License:
This work is licensed under a CC BY 4.0 License. Read Full License
The most recent version of this article is available here.
Background Myostatin (MSTN) and somatostatin (SST) are all involved in regulation the growth of the animals. Animals immunized with these proteins induce the related immune response, which could neutralize the normal inhibitory effects of these two proteins. Here, a recombinant adenovirus expressing MSTN-SST was constructed and used to deliver these two antigen to mice, so as to check the immunize efficacy of these proteins delivered in this way. Results The adenovirus expression fusing protein MSTN-SST was successfully rescued on HEK293A cell as adenovirus related cell pathological change were observed after transfection with the adenovirus genome harboring MSTN-SST gene. The expression of MSTN-SST were confirmed using western blotting and indirect immunofluorescence. Immunization with the recombinant adenovirus once or twice were successfully induce immune response against MSTN-SST and resulted in an improved growth rate and muscle mass. In addition, a booster immunization leaded a better results shown as higher antibody response and mice growth rate. Conclusions Accordingly, adenovirus can be used as a vector for deliver myostatin and somatostatin gene to increase animal growth rate and muscle mass .
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
This is a list of supplementary files associated with this preprint. Click to download.
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