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Research Article
Longhao Jin
Yanbian Hospital: Yanbian University Hospital
Corresponding Author
ORCiD: https://orcid.org/0000-0003-4435-2967
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Autologous bone marrow buffy coat transplantation possesses obvious advantages in the therapy of cartilage defects. However, there is no definite research on the specific effective components of bone marrow buffy coat and its mechanism of cartilage regeneration. Moreover, bone marrow buffy coat is difficult to fix onto the damaged cartilage area. We assessed the effect of using hyaluronic acid(HA) as a gel scaffold mixed with autologous bone marrow buffy coat to fix cartilage defect.
Methods and Materials
We extracted the bone marrow from the anterior superior iliac crest of rabbit, centrifuged to obtain buffy coat, and analyzed the components of buffy coat by ELISA. Buffy coat+fibrin/HA group, MSC+fibrin/HA group, MSC+TGF-β+fibrin/HA group were cultured in vitro and observed by staining. In addition, we made damage to the femoral condyle of rabbits, and divided them into groups: HA group, buffy coat group, buffy coat with HA group. Each group was assessed for cartilage regeneration by visual observation, histological at 4 weeks and 8 weeks, and biochemical analysis at 8 weeks postoperatively. One-way ANOVA and LSD were used for statistic analysis.
Results
Buffy coat have a variety of growth factors, inflammatory factors and anti-inflammatory factors that stimulate the MSCs’ regeneration. Buffy coat can differentiate into cartilage without TGF-β stimulation in vitro. The cartilage regeneration ability of buffy coat and buffy coat+HA is strong, and the combination of buffy coat and gel scaffold HA can make cartilage formation ability more stable in vivo.
Conclusion
MSC and cytokines in buffy coat synergistically promote cartilage regeneration. Gel scaffold HA enhances the effect of buffy coat on cartilage attachment and regeneration of cartilage defects.
Keywords
Cartilage Repair, Buffy Coat, Mesenchymal Stem Cells, Osteoarthritis
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CURRENT STATUS: Under Review
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Posted 22 Feb, 2022
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Received 11 Feb, 2022
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On 30 Jan, 2022
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This preprint is under consideration at Journal of Orthopaedic Surgery and Research. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Longhao Jin
Yanbian Hospital: Yanbian University Hospital
Corresponding Author
ORCiD: https://orcid.org/0000-0003-4435-2967
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 3
Posted 22 Feb, 2022
No community comments so far
Reviews received
Received 11 Feb, 2022
Reviewers invited
Invitations sent on 11 Feb, 2022
Editor assigned
On 30 Jan, 2022
First submitted
On 27 Jan, 2022
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Autologous bone marrow buffy coat transplantation possesses obvious advantages in the therapy of cartilage defects. However, there is no definite research on the specific effective components of bone marrow buffy coat and its mechanism of cartilage regeneration. Moreover, bone marrow buffy coat is difficult to fix onto the damaged cartilage area. We assessed the effect of using hyaluronic acid(HA) as a gel scaffold mixed with autologous bone marrow buffy coat to fix cartilage defect.
Methods and Materials
We extracted the bone marrow from the anterior superior iliac crest of rabbit, centrifuged to obtain buffy coat, and analyzed the components of buffy coat by ELISA. Buffy coat+fibrin/HA group, MSC+fibrin/HA group, MSC+TGF-β+fibrin/HA group were cultured in vitro and observed by staining. In addition, we made damage to the femoral condyle of rabbits, and divided them into groups: HA group, buffy coat group, buffy coat with HA group. Each group was assessed for cartilage regeneration by visual observation, histological at 4 weeks and 8 weeks, and biochemical analysis at 8 weeks postoperatively. One-way ANOVA and LSD were used for statistic analysis.
Results
Buffy coat have a variety of growth factors, inflammatory factors and anti-inflammatory factors that stimulate the MSCs’ regeneration. Buffy coat can differentiate into cartilage without TGF-β stimulation in vitro. The cartilage regeneration ability of buffy coat and buffy coat+HA is strong, and the combination of buffy coat and gel scaffold HA can make cartilage formation ability more stable in vivo.
Conclusion
MSC and cytokines in buffy coat synergistically promote cartilage regeneration. Gel scaffold HA enhances the effect of buffy coat on cartilage attachment and regeneration of cartilage defects.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
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