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Research article
Genetic diversity of Cryptosporidium parvum in neonatal calves in Xinjiang, China
Meng Qi
Tarim University
Corresponding Author
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This work is licensed under a CC BY 4.0 License. Read Full License
Background
The Cryptosporidium causes infection in wide spectrum of vertebrate hosts and is well known for leading to diarrhea and other gastrointestinal illness. To assess Cryptosporidium genetic diversity in neonatal calves and probe the cause for diarrhea of them, a total of 232 fecal samples from neonatal calves in 12 farms in Xinjiang were characterized for the presence of Cryptosporidium .
Results
The prevalence of Cryptosporidium was 38.4% (89/232), and three species were detected with SSU rRNA gene, including C. parvum (n = 88), C. ryanae (n = 9), and C. bovis (n = 1). Prevalence of C . parvum neonatal calves with diarrhea (52.6%, 51/97) was significantly higher than calves without diarrhea (28.1%, 38/135). All the C . parvum -positive samples were analyzed based on gp60 gene, IIdA15G1 (n = 35), IIdA20G1 (n = 21), IIdA14G1 (n = 17), and IIdA19G1 (n = 13) were successfully subtyped in this study.
Conclusions
These data indicated that C . parvum was a major contributor in diarrheal disease in neonatal calves, and C . parvum subtypes from neonatal calves in Xinjiang were high genetic diversity. Additionally, our findings implicating neonatal calves could be a potential source of human Cryptosporidium infection and provide further evidence for the uniqueness of C . parvum IId subtypes in cattle in China.
Keywords
Cryptosporidium, Neonatal calves, Diarrhea, Subtype, Xinjiang
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Peer Review Timeline
CURRENT STATUS: Under Revision
Version 1
Posted 19 Dec, 2019
No community comments so far
Editorial decision: Major revision
On 18 Mar, 2020
Review #2 received
Received 06 Mar, 2020
Reviewer #2 agreed
On 25 Feb, 2020
Review #1 received
Received 31 Jan, 2020
Reviewers invited
Invitations sent on 23 Jan, 2020
Reviewer #1 agreed
On 23 Jan, 2020
Editor assigned
On 17 Dec, 2019
Submission checks complete
On 16 Dec, 2019
Editor invited
On 16 Dec, 2019
First submitted
On 03 Dec, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
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This preprint is under consideration at BMC Microbiology. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Genetic diversity of Cryptosporidium parvum in neonatal calves in Xinjiang, China
Meng Qi
Tarim University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Revision
Version 1
Posted 19 Dec, 2019
No community comments so far
Editorial decision: Major revision
On 18 Mar, 2020
Review #2 received
Received 06 Mar, 2020
Reviewer #2 agreed
On 25 Feb, 2020
Review #1 received
Received 31 Jan, 2020
Reviewers invited
Invitations sent on 23 Jan, 2020
Reviewer #1 agreed
On 23 Jan, 2020
Editor assigned
On 17 Dec, 2019
Submission checks complete
On 16 Dec, 2019
Editor invited
On 16 Dec, 2019
First submitted
On 03 Dec, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
The Cryptosporidium causes infection in wide spectrum of vertebrate hosts and is well known for leading to diarrhea and other gastrointestinal illness. To assess Cryptosporidium genetic diversity in neonatal calves and probe the cause for diarrhea of them, a total of 232 fecal samples from neonatal calves in 12 farms in Xinjiang were characterized for the presence of Cryptosporidium .
Results
The prevalence of Cryptosporidium was 38.4% (89/232), and three species were detected with SSU rRNA gene, including C. parvum (n = 88), C. ryanae (n = 9), and C. bovis (n = 1). Prevalence of C . parvum neonatal calves with diarrhea (52.6%, 51/97) was significantly higher than calves without diarrhea (28.1%, 38/135). All the C . parvum -positive samples were analyzed based on gp60 gene, IIdA15G1 (n = 35), IIdA20G1 (n = 21), IIdA14G1 (n = 17), and IIdA19G1 (n = 13) were successfully subtyped in this study.
Conclusions
These data indicated that C . parvum was a major contributor in diarrheal disease in neonatal calves, and C . parvum subtypes from neonatal calves in Xinjiang were high genetic diversity. Additionally, our findings implicating neonatal calves could be a potential source of human Cryptosporidium infection and provide further evidence for the uniqueness of C . parvum IId subtypes in cattle in China.