Preprint: Please note that this article has not completed peer review.
Research article

Analysis the Detailed Process of Glomerular Capillary Formation Using Immunofluorescence Perform With Ultrathick Section

Chi Liu, Fang Zhang, Yan Wu, Yu Ting, Lu Dai, Furong Li, Chen Jianli, He Xian, Yao Fu, Zhao Jinghong
DOI: 10.21203/rs.2.10191/v1

Abstract

Background: Glomerular capillary formation is one of the fundamental mysteries in renal developmental biology. However, there are still debates on this issue, and its detailed formation process has not been clarified. Results: To resolve this problem, we performed antibody staining with ultra-thick section on embryonic and postnatal mouse kidneys to detect and analyze the development of glomerular capillaries. We found that blood vessel of the fetal kidneys expanded through proliferation and sprouting. During the comma-stage and S-shaped stages, 3-4 capillaries began to bud and migrate into the glomerular cleft, forming a capillary bed in the Bowman’s capsule. Then, the capillary bed expanded into mature glomerular capillary by intussusceptive angiogenesis. The afferent and efferent arterioles were formed through pruning. The distribution of VEGFA in the nephron epithelial cells but not only in podocytes, induced multiple capillaries sprouted into the glomerular cleft. And CXCR4 played an important role in the differentiation and expansion of capillary bed into glomerular capillary. Conclusions: Immunofluorescence performed with ultra-thick section allowed us to investigate the development of complex structure tissues systematically and comprehensively.

Keywords
Glomerular capillary, Ultra-thick sections, Immunofluorescence

Figures

Background

Methods

Results

Discussion

Conclusions

Abbreviations

Declarations

References

Supplementary Files

STATUS: In Review

Comments: 0
PDF Downloads: 0
HTML Views: 6

Integrity Check:

Peer Review Timeline

Version 1

Posted 11 Jun, 2019

  • No community comments so far
  • Review #1 received

    Received 29 Jul, 2019

  • 12 reviewer(s) invited

    Invitations sent on 15 Jul, 2019

  • Reviewer #1 agreed

    On 15 Jul, 2019

  • Editor assigned

    On 27 Jun, 2019

  • Submission checks complete

    On 26 Jun, 2019

  • Editor invited

    On 07 Jun, 2019

More from BMC Developmental Biology

Comments (0)

Comments can take the form of short reviews, notes or questions to the author. Comments will be posted immediately, but removed and moderated if flagged.

Learn more about our company.

Preprint: Please note that this article has not completed peer review.
Research article

Analysis the Detailed Process of Glomerular Capillary Formation Using Immunofluorescence Perform With Ultrathick Section

Chi Liu, Fang Zhang, Yan Wu, Yu Ting, Lu Dai, Furong Li, Chen Jianli, He Xian, Yao Fu, Zhao Jinghong

STATUS: In Review

Comments: 0
PDF Downloads: 0
HTML Views: 6

Integrity Check:

  • Article

  • Peer Review Timeline

  • Related Articles

  • Comments

Abstract

Background: Glomerular capillary formation is one of the fundamental mysteries in renal developmental biology. However, there are still debates on this issue, and its detailed formation process has not been clarified. Results: To resolve this problem, we performed antibody staining with ultra-thick section on embryonic and postnatal mouse kidneys to detect and analyze the development of glomerular capillaries. We found that blood vessel of the fetal kidneys expanded through proliferation and sprouting. During the comma-stage and S-shaped stages, 3-4 capillaries began to bud and migrate into the glomerular cleft, forming a capillary bed in the Bowman’s capsule. Then, the capillary bed expanded into mature glomerular capillary by intussusceptive angiogenesis. The afferent and efferent arterioles were formed through pruning. The distribution of VEGFA in the nephron epithelial cells but not only in podocytes, induced multiple capillaries sprouted into the glomerular cleft. And CXCR4 played an important role in the differentiation and expansion of capillary bed into glomerular capillary. Conclusions: Immunofluorescence performed with ultra-thick section allowed us to investigate the development of complex structure tissues systematically and comprehensively.

Figures

Background

Methods

Results

Discussion

Conclusions

Abbreviations

Declarations

References

Learn more about our company.