Preprint: Please note that this article has not completed peer review.

Prevalence of mobile antimicrobial resistance genes carrying and toxin producing pathogens in retail beef and mutton

ying liu, Yifang Cui, Wenjing Peng, Baoyong Huang, Lichao Ma, Min Zheng, Shuangyang Ding, Kui Zhu
DOI: 10.21203/rs.2.12408/v1

Abstract

Background

Food safety has always been the global issue. In addition to food poisoning problems caused by toxin-producing strains, the arising of widely spread antimicrobial resistant bacteria have become a major issue that impacts food safety. The purpose of this study is to assess the safety of retail meat in the last link of sales.

Results

A total number of 134 samples were collected and 674 strains were isolated. There are different bacterial compositions distributed in meat, environmental and human-derived samples. The major pathogens in meat and environmental or human samples are Klebsiella spp. and Staphylococcus spp.. The resistance to amoxicillin + clavulanate (with the resistance rate of 46.78%), tetracycline (44.66%) and erythromycin (32.73%) are major resistant phenotypes of the isolates. According to the whole genome analysis, two K. pneumoniae strains harboring the extended spectrum beta-lactamase genes which are located on mobile elements and two Aeromonas hydrophila isolates carrying mcr-7.1 like genes have been detected. The major toxin genes of Bacillus cereus, and adhesion or invasion related virulence factors were also shared among the isolates.

Conclusion

There are different pathogens distributed in meat, environment and human source at the final stage of meat consumption. The mobile ARGs are prevalent in strains isolates from meat samples. And toxin-producing strains can be isolated from human source. These factors consist potential risk for public health and need attention.

Keywords
retail meat, foodborne pathogens, antimicrobial resistance, virulence factors

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Preprint: Please note that this article has not completed peer review.

Prevalence of mobile antimicrobial resistance genes carrying and toxin producing pathogens in retail beef and mutton

ying liu, Yifang Cui, Wenjing Peng, Baoyong Huang, Lichao Ma, Min Zheng, Shuangyang Ding, Kui Zhu

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Abstract

Background

Food safety has always been the global issue. In addition to food poisoning problems caused by toxin-producing strains, the arising of widely spread antimicrobial resistant bacteria have become a major issue that impacts food safety. The purpose of this study is to assess the safety of retail meat in the last link of sales.

Results

A total number of 134 samples were collected and 674 strains were isolated. There are different bacterial compositions distributed in meat, environmental and human-derived samples. The major pathogens in meat and environmental or human samples are Klebsiella spp. and Staphylococcus spp.. The resistance to amoxicillin + clavulanate (with the resistance rate of 46.78%), tetracycline (44.66%) and erythromycin (32.73%) are major resistant phenotypes of the isolates. According to the whole genome analysis, two K. pneumoniae strains harboring the extended spectrum beta-lactamase genes which are located on mobile elements and two Aeromonas hydrophila isolates carrying mcr-7.1 like genes have been detected. The major toxin genes of Bacillus cereus, and adhesion or invasion related virulence factors were also shared among the isolates.

Conclusion

There are different pathogens distributed in meat, environment and human source at the final stage of meat consumption. The mobile ARGs are prevalent in strains isolates from meat samples. And toxin-producing strains can be isolated from human source. These factors consist potential risk for public health and need attention.

Figures

Introduction

Materials and Methods

Results

Discussion

Declarations

References

Tables

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