Setting up the Talos Arctica electron microscope and Gatan K2 direct detector for high-resolution cryogenic single-particle data acquisition
It has been determined that a Talos Arctica transmission electron microscope (TEM) is capable of resolving frozen-hydrated macromolecules of various sizes and internal symmetries to better than 3 Å resolution using single particle methodologies. This protocol describes how to maximize parallel illumination conditions in a Talos Arctica TEM operating at 200 keV for high-resolution cryogenic data acquisition for structure determination.
Posted 12 Oct, 2017
Setting up the Talos Arctica electron microscope and Gatan K2 direct detector for high-resolution cryogenic single-particle data acquisition
Posted 12 Oct, 2017
It has been determined that a Talos Arctica transmission electron microscope (TEM) is capable of resolving frozen-hydrated macromolecules of various sizes and internal symmetries to better than 3 Å resolution using single particle methodologies. This protocol describes how to maximize parallel illumination conditions in a Talos Arctica TEM operating at 200 keV for high-resolution cryogenic data acquisition for structure determination.