Preprint: Please note that this article has not completed peer review.

Setting up the Talos Arctica electron microscope and Gatan K2 direct detector for high-resolution cryogenic single-particle data acquisition

Gabriel C. Lander, Mark A. Herzik, Jr., Mengyu Wu
DOI: 10.1038/protex.2017.108

Abstract

It has been determined that a Talos Arctica transmission electron microscope (TEM) is capable of resolving frozen-hydrated macromolecules of various sizes and internal symmetries to better than 3 Å resolution using single particle methodologies. This protocol describes how to maximize parallel illumination conditions in a Talos Arctica TEM operating at 200 keV for high-resolution cryogenic data acquisition for structure determination.

Keywords
cryo-EM, electron microscopy, 200 kev, Talos Arctica, Gatan K2

Introduction

Reagents

Equipment

Procedure

References

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Preprint: Please note that this article has not completed peer review.

Setting up the Talos Arctica electron microscope and Gatan K2 direct detector for high-resolution cryogenic single-particle data acquisition

Gabriel C. Lander, Mark A. Herzik, Jr., Mengyu Wu

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Abstract

It has been determined that a Talos Arctica transmission electron microscope (TEM) is capable of resolving frozen-hydrated macromolecules of various sizes and internal symmetries to better than 3 Å resolution using single particle methodologies. This protocol describes how to maximize parallel illumination conditions in a Talos Arctica TEM operating at 200 keV for high-resolution cryogenic data acquisition for structure determination.

Introduction

Reagents

Equipment

Procedure

References

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