Preprint: Please note that this article has not completed peer review.
Method Article

Preparation of soft tissue samples for synchrotron radiation microtomography and nanotomography

Ryuta Mizutani, Rino Saiga

Abstract

In synchrotron radiation microtomography and nanotomography, images are taken while rotating the sample and irradiating it with a high x-ray flux. The structure of the sample must remain fixed at micrometer to nanometer precision throughout the image acquisition process. Hence, in order to visualize soft tissue structures by using synchrotron radiation sources, tissue samples should be prepared by taking account of a number of factors that affect the image quality. The procedure described in this protocol is based on our studies reporting 3D structures of biological samples of human, mouse, zebra fish, fruit fly, and Escherichia coli. Soft tissue samples prepared according to this procedure can be three-dimensionally visualized at a resolution up to 100 nm.

Keywords
synchrotron radiation, microtomography, micro-CT, nanotomography, nano-CT, soft tissue

Figures

Introduction

Reagents

Equipment

Procedure

Troubleshooting

Anticipated Results

References

Acknowledgements

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Preprint: Please note that this article has not completed peer review.
Method Article

Preparation of soft tissue samples for synchrotron radiation microtomography and nanotomography

Ryuta Mizutani, Rino Saiga

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Abstract

In synchrotron radiation microtomography and nanotomography, images are taken while rotating the sample and irradiating it with a high x-ray flux. The structure of the sample must remain fixed at micrometer to nanometer precision throughout the image acquisition process. Hence, in order to visualize soft tissue structures by using synchrotron radiation sources, tissue samples should be prepared by taking account of a number of factors that affect the image quality. The procedure described in this protocol is based on our studies reporting 3D structures of biological samples of human, mouse, zebra fish, fruit fly, and Escherichia coli. Soft tissue samples prepared according to this procedure can be three-dimensionally visualized at a resolution up to 100 nm.

Figures

Introduction

Reagents

Equipment

Procedure

Troubleshooting

Anticipated Results

References

Acknowledgements

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