Remazol Brilliant Blue R (RBBR) is a widely used carcinogenic and toxic dye. This study focused on RBBR dye from aqueous solution using potassium permanganate, cetyltrimethylammonium bromide (CTAB) modified, and unmodified Yarrowia lipolytica biomass as biosorbent. RBBR dye biosorption studies were carried out as a function of pH, initial dye concentration, biosorbent dose, contact time, and temperature. The pH of the aqueous solution strongly influenced the biosorption percent of RBBR dye. The highest dye biosorption capacity yield was obtained at pH 2-3 as well as above pH 3, very low yield biosorption of RBBR was observed. No differences were found between chemically modified and unmodified biomass in terms of RBBR dye biosorption capacity. In the first 15 min, almost 50% RBBR dye was removed from the solution and reached equilibrium within,180 min at pH 2. Biosorption isotherm obeyed Langmuir isotherm model and pseudo-second-order kinetic model.