1. Wang Z, Wang X, Hao G, Chen Z, Zhang L, Shao L, Tian Y, Dong Y, Zheng C,Kang Y, Gao R. A national study of the prevalence and risk factors associated with peripheral arterial disease from China: The China Hypertension Survey, 2012–2015. International Journal of Cardiology. 2019; 275, 165–170.
2. Adam DJ, Beard JD, Cleveland T, Bell J, Bradbury AW, Forbes JF. BASIL trial participants. Bypass versus angioplasty in severe ischemia of the leg (BASIL): multicentre, randomized controlled trial. Lancet. 2005;366,1925-34.
3. Cui S, Guo L, Li X, Gu Y, Fu J, Dong L, Song H, Chen X, Lu Y, Hu C, Xiao F, Zhu D, Wu Z, Zhang Q. Clinical Safety and Preliminary Efficacy of Plasmid pUDK-HGF Expressing Human Hepatocyte Growth Factor (HGF) in Patients with Critical Limb Ischemia. Eur J Vasc Endovasc Surg (2015) 50,494-501.
4. Shigematsu H, Yasuda K, Iwai T, Sasajima T, Ishimaru S, Ohashi Y, Yamaguchi T, Ogihara T, Morishita R, Randomized, double-blind, placebo controlled clinical trial of hepatocyte growth factor plasmid for critical limb ischemia. Gene Ther 2010; 17:1152–1161.
5. Powell RJ, Goodney P, Mendelsohn FO, Moen EK, Annex BH, HGF-0205 Trial Investigators. Safety and efficacy of patient specific intramuscular injection of HGF plasmid gene therapy on limb perfusion and wound healing in patients with ischemic lower extremity ulceration: results of the HGF-0205 trial. J Vasc Surg 2010; 52: 1525–1530.
6. Morishita R, Makino H, Aoki M, Hashiya N, Yamasaki K, Azuma J, Taniyama Y, Sawa Y, Kaneda Y, Ogihara T, Phase I/IIa clinical trial of therapeutic angiogenesis using hepatocyte growth factor gene transfer to treat critical limb ischemia. Arterioscler Thromb Vasc Biol 2011; 31: 713–720.
7. Shigematsu H, Yasuda K, Sasajima T, Takano T, Miyata T, Ohta T, Tanemoto K, Obitsu Y, Iwai T, Ozaki S, Ogihara T, Morishita R; Transfection of human HGF plasmid DNA improves limb salvage in Buerger’s disease patients with critical limb ischemia. Int Angiol 2011; 30: 140–149.
8. Powell RJ, Simons M, Mendelsohn FO, Daniel G, Henry TD, Koga M, Morishita R, Annex BH, Result of a double-blind, placebo-controlled study to assess the safety of intramuscular injection of hepatocyte growth factor plasmid to improve limb perfusion in patients with critical limb ischemia. Circulation. 2008;118:58-65.
9. Makino H, Aoki M, Hashiya N, Yamasaki K, Azuma J, Sawa Y, Kaneda Y, Ogihara T, Morishita R, Long-term follow-up evaluation of results from clinical trial using hepatocyte growth factor gene to treat severe peripheral arterial disease. Arterioscler Thromb Vasc Biol. 2012; 32:2503-9.
10. Ajroud-Driss S, Christiansen M, Allen JA, Kessler JA. Phase 1/2 open-label dose-escalation study of plasmid DNA expressing two isoforms of hepatocyte growth factor in patients with painful diabetic peripheral neuropathy. Mol Ther. 2013; 21:1279-86.
11. Gu YQ, Zhang J, Guo LR, Cui S, Li X, Ding D, Kim Jk, Ho SH, Hahn W, Kim S, A phase I clinical study of naked DNA expressing two isoforms of hepatocyte growth factor to treat patients with critical limb ischemia. J Gene Med 2011; 13: 602–610.
12. Henry TD, Hirsh AT, Goldman J, Wang YL, Lips DL, McMillan WD, Duval S, Bigg TA, Keo HH, Safety of a non-viral plasmid-encoding dual isoforms of hepatocyte growth factor in critical limb ischemia patients: a phase I study. Gene Ther 2011; 18:788–794.
13. Ha XQ, Li YM, Bi JJ, Zhang QL, Wu ZZ. Plasmid pUDKH gene therapy of rat acute hind limb ischemia: an experimental study. Natl Med J China. 2003; 83: 1521-1524.
14. Ha XQ, Yuan B, Li YM, Yao SL, Wu ZZ. Application of gene gun in treatment of occlusive vascular diseases in rats. Let Biotech. 2001; 12:266-269.
15. Ha XQ, Ren JP, Bi JJ, Zhang JH, Zhang QL, Wu ZZ. Human hepatocyte growth factor for gene therapy of peripheral arterial occlusive disease in dogs. Chin Sci Bull. 2003; 48:571-574
16. Yang YF, Wang RL, Liu YX, Wang H, Bi JJ, Zhang QL, Wu ZZ, LS Wang. Distribution of recombinant plasmid carrying hepatocyte growth factor gene in rat. Chin Med Biotech. 2008; 3: 273-278.
17. Bi JJ, Wu DL, Zhang JH, Jin JD, Wang YF, Gong P, Wang XN, Zhang QL, Wu ZZ. Safety evaluation on the treatment of periphery artery disease by plasmid. Let Biotech. 2002; 13: 443-444.
18. Hu C, Lu Y, Chen X, Wu Z, Zhang Q. Gene transfer of a naked plasmid (pUDK- HGF) encoding human hepatocyte growth factor attenuates skin/muscle incision and retraction-induced chronic post-surgical pain in rats. J Gene Med. 2017 ;19 (12)
19. Hu C, Lu Y, Cheng X, Cui Y, Wu Z, Zhang Q. Gene therapy for neuropathic pain induced by spared nerve injury with naked plasmid encoding hepatocyte growth factor. Eur J Pain. 2018: 22(5): 961-972.
20. Hauser CJ., Shoemaker W C., Use of a Transcutaneous P02 regional perfusion index to quantify tissue perfusion in peripheral vascular disease, Ann. Surg, 1983;197(3): 337-343