Herein, release of gabapentin molecules from gold nanoparticles (Au NPs) is studied by using two kinds of laser beams. The first is a green laser, which matches well with the frequency of the Au NPs’ SPR and the second is a red laser that has the longest penetration depth in the tissue. Au NPs are synthesized by the Turkevich method and characterized with different techniques such as FT-IR, XRD, TEM, UV-VIS, and DLS. Then, the effects of two green and red lasers as well as temperature are investigated in the delivery of gabapentin drug molecules from Au NPs. Slices of chicken breast muscle with different thicknesses are also used in order to disclose an experimental condition close to the in vivo study. The drug release amount is, then, correlated with the return of the absorption-peak to its primary position. The effects of temperature and penetration depth of the laser beam investigated on the UV-VIS spectrum of Au NPs and the results are compared to attain the best release conditions. It is found that although the green laser is better than the red one to stimulate the SPR of Au NPs, however, the red laser is preferred for the drug delivery purposes due to its greater penetration depth. Also, increasing the temperature of the capped nanocolloid enhances the rate of drug release by the laser irradiation. Therefore, this investigation is helpful to understand the effect of temperature and a red laser beam on different metallic nanoparticles carrying drug molecules.