This study investigated the potential use of Eucalyptus Bark (EB) powder as an adsorbent in batch mode experiments for removal of Cu2+ from Ezana (Meli) wastewater. The discharge of untreated gold mining wastewater contaminated by Cu (II), which is threatening ecosystems and carcinogenic to the human. Since the removal by using adsorption method is cost effective and environmentally friendly, it has been widely studied by many researchers. Characterizations of Eucalyptus Bark were analyzed using proximate analysis, Fourier transform infrared (FTIR) and X-ray diffractometer (XRD). Various characterization techniques showed that the effluent discharged from the factory contains: total suspended solid (TSS), turbidity, Electrical conductivity (EC), Total dissolved solid (TDS), COD, Temperature, pH, cyanide WAD with <11°C (ppm). Atomic absorption spectroscopy study indicated that heavy metals found in the wastewater were in the order Fe2+> Cu (II) >Pb (II) >Mn> Cr (VI) >Zn > Co > Ni > Cd in ppm. The selected parameters were pH, adsorbent dosage and contacting time. The highest percentage of Cu (II) removal achieved was 92%. In this study, the adsorption data were well-fitted to the Langmuir isotherm model.