This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research
Daming Wu
Nanjing Medical University Second Affiliated Hospital
Corresponding Author
ORCiD: https://orcid.org/0000-0002-2326-9630
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Silver (Ag) and/or zinc (Zn) incorporated mesoporous calcium-silicate nanoparticles (MCSNs) have good physicochemical characteristics and can be advanced materials for root canal filling. This study was to analyze the antibacterial properties and mechanism of Ag/Zn-MCSNs with different percentage of Ag and Zn.
Methods: The antibacterial properties and the cytotoxicity of them were evaluated. Human root canals were inoculated with E. faecalis for 4 weeks to establish bacterial biofilm model. The E. faecalis biofilms were treated with MCSNs, Ag-MCSNs, Zn-MCSNs, Ag/Zn-MCSNs, calcium hydroxide (CH) and mineral trioxide aggregate (MTA) for 7 days. The ultrastructure and distribution of viable bacteria of the specimens were evaluated using SEM and CLSM. Human root canals were pretreated with CH, MCSNs, Ag-MCSNs, Zn-MCSNs and Ag/Zn-MCSNs, then the root canals were immersed in E. faecalis suspension for 7 days. The adhesion and colonization of E. faecalis on the root canal walls were observed using SEM and CLSM. Endocytosis of E. Faecalis treated by Ag-MCSNs and Ag/Zn-MCSNs were observed using TEM.
Results: The MCSNs containing Ag showed better antibacterial properties than MCSNs and Zn-MCSNs (P<0.05). MCSNs, Ag/Zn(1:1)-MCSNs and Ag/Zn(1:9)-MCSNs showed no obvious cytotoxicity (P>0.05), while Ag-MCSNs and Ag/Zn(9:1)-MCSNs showed cytostatic effects. Zn-MCSNs slightly promote cell proliferation (P<0.05). The MCSNs containing Ag showed antibacterial abilities against E. faecalis biofilm in different degree, and can adhere to dentin surfaces to get a continuous antibacterial effect, but MTA, MCSNs and Zn-MCSNs could not disrupt the bacterial biofilm obviously. The Ag-MCSNs and Ag/Zn-MCSNs release Ag+ and destroy the cell membranes to kill bacteria.
Conclusions: The antibacterial effects and cytotoxicity of Ag/Zn-MCSNs are related to Ag content, while Zn reduce cytotoxicity and promote cell proliferation.
Keywords
Antibacterial property, Bacterial biofilm, Mesoporous calcium-silicate nanoparticles, Silver, Zinc
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research
Daming Wu
Nanjing Medical University Second Affiliated Hospital
Corresponding Author
ORCiD: https://orcid.org/0000-0002-2326-9630
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 30 Dec, 2019
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Nanoscience
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Silver (Ag) and/or zinc (Zn) incorporated mesoporous calcium-silicate nanoparticles (MCSNs) have good physicochemical characteristics and can be advanced materials for root canal filling. This study was to analyze the antibacterial properties and mechanism of Ag/Zn-MCSNs with different percentage of Ag and Zn.
Methods: The antibacterial properties and the cytotoxicity of them were evaluated. Human root canals were inoculated with E. faecalis for 4 weeks to establish bacterial biofilm model. The E. faecalis biofilms were treated with MCSNs, Ag-MCSNs, Zn-MCSNs, Ag/Zn-MCSNs, calcium hydroxide (CH) and mineral trioxide aggregate (MTA) for 7 days. The ultrastructure and distribution of viable bacteria of the specimens were evaluated using SEM and CLSM. Human root canals were pretreated with CH, MCSNs, Ag-MCSNs, Zn-MCSNs and Ag/Zn-MCSNs, then the root canals were immersed in E. faecalis suspension for 7 days. The adhesion and colonization of E. faecalis on the root canal walls were observed using SEM and CLSM. Endocytosis of E. Faecalis treated by Ag-MCSNs and Ag/Zn-MCSNs were observed using TEM.
Results: The MCSNs containing Ag showed better antibacterial properties than MCSNs and Zn-MCSNs (P<0.05). MCSNs, Ag/Zn(1:1)-MCSNs and Ag/Zn(1:9)-MCSNs showed no obvious cytotoxicity (P>0.05), while Ag-MCSNs and Ag/Zn(9:1)-MCSNs showed cytostatic effects. Zn-MCSNs slightly promote cell proliferation (P<0.05). The MCSNs containing Ag showed antibacterial abilities against E. faecalis biofilm in different degree, and can adhere to dentin surfaces to get a continuous antibacterial effect, but MTA, MCSNs and Zn-MCSNs could not disrupt the bacterial biofilm obviously. The Ag-MCSNs and Ag/Zn-MCSNs release Ag+ and destroy the cell membranes to kill bacteria.
Conclusions: The antibacterial effects and cytotoxicity of Ag/Zn-MCSNs are related to Ag content, while Zn reduce cytotoxicity and promote cell proliferation.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10
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