Combined results obtained from phylogenetic, genomic, and chemotaxonomic analyses made it reasonable to assign strain BSSL-BM10T as a member of the genus Devosia (Fig. 1; Figs. S1, S2 and S3; Table 1). Strain BSSL-BM10T was distinguished from type strains of D. naphthalenivorans and D. riboflavina by differences in several phenotypic characteristics, including nitrate reduction, acid production from D-glucose, utilization of some substrates, susceptibility to some antibiotics, and activities of some enzymes (Table 2). Distinguished phenotypic properties, 16S rRNA gene sequence similarities, and genetic distinctiveness based on ANI values and dDDH values suggest that strain BSSL-BM10T is separated from recognized species of genus Devosia (Chun et al. 2018; Goris et al. 2007; Konstantinidis and Tiedje 2005; Richter and Rosselló-Móra 2009). Based on polyphasic taxonomic data presented, strain BSSL-BM10T is considered to represent a novel Devosia species, for which we propose the name Devosia litorisediminis sp. nov.
Description of Devosia litorisediminis sp. nov.
Devosia litorisediminis (li.to.ri.se.di’mi.nis. L. n. litus –oris the seashore, coast; L. n. sedimen –inis sediment; N.L. gen. n. litorisediminis of a coastal sediment, tidal flat sediment).
Cells are rod-shaped measuring approximately 0.3-0.8 µm in diameter and 0.8-4.0 µm in length. Gram-staining reaction is negative. Spore is not formed. No flagellum is found. Colonies on TSA are circular, convex, smooth, glistening, grayish yellow in colour, and 0.5-1.0 mm in diameter after incubation at 30°C for 5 days. Grows optimally at 30°C and pH 7.0-8.0. Growth occurs at 4 °C to 37°C, but not at 40°C and occurs at pH 5.0, but not at pH 4.5. Growth occurs in the presence of 0.5-5.0% (w/v) NaCl with an optimum of approximately 1.0-2.0% (w/v) NaCl. Anaerobic growth does not occur on TSA or TSA supplemented with nitrate. It is catalase- and oxidase-positive. Nitrate is not reduced to nitrite. Aesculin, hypoxanthine, urea, and xanthine are hydrolyzed, while casein, gelatin, starch, Tween 80, and L-tyrosine are not. L-Arabinose, D-galactose, D-glucose, maltose, D-cellobiose, D-fructose, D-mannose, sucrose, D-trehalose, D-xylose, acetate, citrate, succinate, L-malate, pyruvate, and salicin are utilized as carbon and energy sources, but benzoate, formate, and L-glutamate are not. Susceptible to ampicillin (10 µg), carbenicillin (100 µg), chloramphenicol (100 µg), neomycin (30 µg), novobiocin (5 µg), oleandomycin (15 µg), penicillin G (20 IU), and tetracycline (30 µg), but resistant to cephalothin (30 µg), gentamicin (30 µg), kanamycin (30 µg), lincomycin (15 µg), polymyxin B (100 IU), and streptomycin (50 µg). In assays with API 20NE system, it is positive for activity of urease and hydrolysis of 4-nitrophenyl-β-D-galactopyranoside, but negative for indole production, arginine dihydrolase, and acid production from D-glucose and gelatin hydrolysis. In assays with the API ZYM system, activities of esterase (C4), esterase lipase (C8), leucine and valine arylamidases, trypsin, alkaline and acid phosphatases, naphthol-AS-BI-phosphohydrolase, β-galactosidase, α-glucosidase, β-glucosidase, N-acetyl-β-glucosaminidase, and α-fucosidase are present, but activities of other enzymes are absent. The predominant ubiquinone is Q-10. The major fatty acids (> 10% of total fatty acids) are 11-methyl C18:1 ω7c, C18:1 ω7c, summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), and C16:0. The major polar lipids are phosphatidylglycerol and two unidentified glycolipids.
The type strain, BSSL-BM10T (= KACC 21633T = NBRC 115152T), was isolated from a sand dune at Boryeong on the Yellow Sea, Republic of Korea. The DNA G+C content of the type strain is 60.9% (from genome sequence data). The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence and GenBank accession number for the whole genome shotgun sequence of strain BSSL-BM10T are MN872411and JAGXTP000000000, respectively.