Transcriptome analysis and molecular mechanism of linseed (Linum usitatissimum L.) drought tolerance under repeated drought using single-molecule long-read sequencing
Background: Oil flax (linseed, Linum usitatissimum L.) is one of the most important oil crops., However, the increases in drought resulting from climate change have dramatically reduces linseed yield and quality, but very little is known about how linseed coordinates the expression of drought resistance gene in response to different level of drought stress (DS) on the genome-wide level.
Results: To explore the linseed transcriptional response of DS and repeated drought (RD) stress, we determined the drought tolerance of different linseed varieties. Then we performed full-length transcriptome sequencing of drought-resistant variety (Z141) and drought-sensitive variety (NY-17) under DS and RD stress at the seedling stage using single-molecule real-time sequencing and RNA-sequencing. Gene Ontology (GO) and reduce and visualize GO (REVIGO) enrichment analysis showed that upregulated genes of Z141 were enriched in more functional pathways related to plant drought tolerance than those of NY-17 were under DS. In addition, 4436 linseed transcription factors were identified, and 1190 were responsive to stress treatments. Moreover, protein-protein interaction (PPI) network analysis showed that the proline biosynthesis pathway interacts with stress response genes through RAD50 (DNA repair protein 50) interacting protein 1 (RIN-1). Finally, proline biosynthesis and DNA repair structural gene expression patterns were verified by RT- PCR.
Conclusions: The drought tolerance of Z141 may be related to its upregulation of drought tolerance genes under DS. Proline may play an important role in linseed drought tolerance by maintaining cell osmotic and protecting DNA from ROS damage. In summary, this study provides a new perspective to understand the drought adaptability of linseed.
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Figure S1. Bubble diagram showing the GO classification of differentially expressed transcripts between DS and RD in Z141 or NY-17. (a, b) GO terms of downregulated genes overlapping between DS and RD in Z141 (a) or NY-17 (b). (c-f) GO terms of genes up- (c, d) or downregulated (e, f) in only Z141 under DS or RD, respectively. (g-j) GO terms of genes up- (g, h) or downregulated (i, j) in only NY-17 under DS or RD respectively.
Figure S2. Bubble diagram showing the GO classification of differentially expressed transcripts between Z141 and NY-17 under DS or RD treatment. (a, b) GO terms of downregulated genes overlapping between Z141 and NY-17 under DS (a) or RD (b) treatment. (c-f) GO terms of genes up- (c, d) or downregulated (e, f) in Z141 or NY-17 under only DS. (g-j) GO terms of genes up- (g, h) or downregulated (i, j) in Z141 or NY-17 under only RD.
Figure S3. Tree diagram showing the REVIGO classification of up- or downregulated differentially expressed transcripts in Z141 or NY-17 under DS or RD. (a, b) REVIGO classification of up- (a) and downregulated (b) genes in Z141 under RD stress. (c, d) REVIGO classification of up- (c) and downregulated (d) genes in NY-17 under DS. (e, f) REVIGO classification of up- (e) and downregulated (f) genes in NY-17 under RD stress.
Figure S4. MapMan visualization of drought stress-responsive DEGs in Z141 (b) and NY-17 (a, c) under DS and RD stress, respectively. The up- and downregulated DEGs are represented in red and blue colour. The Colour brightness indicates the degree of difference, as shown in the scale on the right.
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On 26 Dec, 2019
Transcriptome analysis and molecular mechanism of linseed (Linum usitatissimum L.) drought tolerance under repeated drought using single-molecule long-read sequencing
Posted 31 Dec, 2020
On 09 Feb, 2021
On 10 Jan, 2021
On 14 Dec, 2020
On 14 Dec, 2020
On 14 Dec, 2020
On 11 Dec, 2020
Received 09 Dec, 2020
On 21 Nov, 2020
Invitations sent on 21 Nov, 2020
On 21 Nov, 2020
On 21 Nov, 2020
On 21 Nov, 2020
On 29 Oct, 2020
On 13 Oct, 2020
Received 13 Oct, 2020
Received 25 Aug, 2020
On 08 Aug, 2020
Invitations sent on 05 Aug, 2020
On 05 Aug, 2020
Received 05 Aug, 2020
On 14 Jul, 2020
On 13 Jul, 2020
On 13 Jul, 2020
Received 27 May, 2020
On 27 May, 2020
Received 26 May, 2020
On 29 Apr, 2020
On 22 Apr, 2020
Received 28 Jan, 2020
Invitations sent on 08 Jan, 2020
On 08 Jan, 2020
On 30 Dec, 2019
On 29 Dec, 2019
On 29 Dec, 2019
On 26 Dec, 2019
Background: Oil flax (linseed, Linum usitatissimum L.) is one of the most important oil crops., However, the increases in drought resulting from climate change have dramatically reduces linseed yield and quality, but very little is known about how linseed coordinates the expression of drought resistance gene in response to different level of drought stress (DS) on the genome-wide level.
Results: To explore the linseed transcriptional response of DS and repeated drought (RD) stress, we determined the drought tolerance of different linseed varieties. Then we performed full-length transcriptome sequencing of drought-resistant variety (Z141) and drought-sensitive variety (NY-17) under DS and RD stress at the seedling stage using single-molecule real-time sequencing and RNA-sequencing. Gene Ontology (GO) and reduce and visualize GO (REVIGO) enrichment analysis showed that upregulated genes of Z141 were enriched in more functional pathways related to plant drought tolerance than those of NY-17 were under DS. In addition, 4436 linseed transcription factors were identified, and 1190 were responsive to stress treatments. Moreover, protein-protein interaction (PPI) network analysis showed that the proline biosynthesis pathway interacts with stress response genes through RAD50 (DNA repair protein 50) interacting protein 1 (RIN-1). Finally, proline biosynthesis and DNA repair structural gene expression patterns were verified by RT- PCR.
Conclusions: The drought tolerance of Z141 may be related to its upregulation of drought tolerance genes under DS. Proline may play an important role in linseed drought tolerance by maintaining cell osmotic and protecting DNA from ROS damage. In summary, this study provides a new perspective to understand the drought adaptability of linseed.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10
Figure 11
Figure 12