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Research article
Transcriptomic Profiling of Cotton Leaves in Response to Cotton Aphid Damage
Jianmin Zhang
Yangtze University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-7773-4470
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Cotton aphids (Aphis gossypii Glover) are regarded as one of the most harmful insect pests for cotton production. They are usually capable of causing severe yield loss through sucking cotton liquids, secreting honeydew and transmitting plant viral diseases. However, the molecular mechanism of the interaction between cotton and cotton aphids remains unclear currently. Therefore, the RNA-Seq study of cotton leaves was performed in response to cotton aphid damage at different time points (0 h, 6 h, 12 h, 24 h, 48 h and 72 h).
Results
A total of 9, 103 new genes were identified, and 7, 510 of them were annotated functionally. Based on the comparison results, the gene expression was analyzed according to the expression amount of genes in different samples. 24,793 differentially expressed genes were authenticated in all and their functional annotation and enrichment analysis were conducted. Compared with 0 h (without aphid damage, CK), the amount of down-regulated DEGs was largely more than that of the up-regulated genes at different time points under cotton aphid attack except for 48h. As revealed by the functional annotation of DEGs, these genes were involved in all kinds of plant biological process, including various resistance to abiotic and biotic stress, hormone metabolism, signaling transduction and transcriptional regulation.
Conclusions
The results revealed the molecular mechanism of the interaction between cotton and cotton aphids and would facilitate the development of plant aphid resistant cultivars.
Keywords
Gossypium hirsutum, Aphis gossypii Glover, RNA-Seq, Differentially expressed genes, Transcription factors, Aphid resistant genes
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Due to technical limitations, table 1 is only available as a download in the supplemental files section.
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This is a preprint. It has not completed peer review.
Research article
Transcriptomic Profiling of Cotton Leaves in Response to Cotton Aphid Damage
Jianmin Zhang
Yangtze University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-7773-4470
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 09 Jan, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Epigenetics & Genomics
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Cotton aphids (Aphis gossypii Glover) are regarded as one of the most harmful insect pests for cotton production. They are usually capable of causing severe yield loss through sucking cotton liquids, secreting honeydew and transmitting plant viral diseases. However, the molecular mechanism of the interaction between cotton and cotton aphids remains unclear currently. Therefore, the RNA-Seq study of cotton leaves was performed in response to cotton aphid damage at different time points (0 h, 6 h, 12 h, 24 h, 48 h and 72 h).
Results
A total of 9, 103 new genes were identified, and 7, 510 of them were annotated functionally. Based on the comparison results, the gene expression was analyzed according to the expression amount of genes in different samples. 24,793 differentially expressed genes were authenticated in all and their functional annotation and enrichment analysis were conducted. Compared with 0 h (without aphid damage, CK), the amount of down-regulated DEGs was largely more than that of the up-regulated genes at different time points under cotton aphid attack except for 48h. As revealed by the functional annotation of DEGs, these genes were involved in all kinds of plant biological process, including various resistance to abiotic and biotic stress, hormone metabolism, signaling transduction and transcriptional regulation.
Conclusions
The results revealed the molecular mechanism of the interaction between cotton and cotton aphids and would facilitate the development of plant aphid resistant cultivars.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Due to technical limitations, table 1 is only available as a download in the supplemental files section.