Microbial destruction of organochlorine pollutants is the one of the most effective approaches, the safest and promising methods for remediation the environment from pollution. This study presents strains of microorganisms that destroy hexachlorocyclohexane: Stenotrophomonas maltophili a IMV B-7288, Pseudomonas putida IMV B-7289 and Bacillus megaterium IMV B-7287 - new selected destructors of organochlorine pesticide hexachlorocyclohexane. Their advantages and features are considered, namely - exclusively of natural origin - microorganisms are isolated from places of total pollution. The studied strains are characterized by high resistance to the HCH contaminant (in the range of 100-1000 mg / l) and the ability to decompose in soil and liquid medium. We have found that strains B. megaterium IMV B-7287 and P. putida IMV B-7289 showed a high efficiency of destruction of HCH in laboratory studies when cultivated on a chlorine-free MM medium at 70.4-89.3% from initial content. For S. maltophilia IMV B-7288 has been found that the ability to degrade HCH-isomers depends on the season a little and it was at maximum in the summer for every studied HCH-isomer: 61.6–82.1% from initial amount. The investigated strains are promising for further work to create microbial compositions with the aim to provide an effictive destruction of HCH-isomers complex.