Description of Nakazawaea odontotermitis f.a. sp. nov. (S. Tiwari, B.C. Behera and A. Baghela)
Nakazawaea odontotermitis (odon.to.ter′mi.tis. N.L. gen. n. odontotermitis of the host termite genus Odontotermes).
After 10 days at 25°C in YM agar, the colony is circular, white with an opaque sector, shiny, smooth, butyrous with an entire margin (Fig. 2a). After three days at 25°C in YM broth, vegetative cells are globose to subglobose (2.5–5 × 3–5 µm) and occur singly or in groups (Fig. 2b). Budding is multilateral, though predominantly polar (Fig. 2b & c). Hyphae or pseudohyphae are not observed on Dalmau (Corn Meal Agar) plates in slide culture, even after 21 days at 25°C. Ascospores are not produced on YPD agar, 5% malt extract agar, McClary’s acetate agar, Gorodkowa agar or cornmeal agar after 4 weeks at 15 and 25°C. Fermentation of glucose is positive, but negative for D-galactose, sucrose, maltose, raffinose, lactose, α α-trehalose, xylose and cellobiose. The following carbon compounds are assimilated: D-glucose, D-galactose (weak), L-sorbose, sucrose, maltose, cellobiose (slow and weak), salicin, α α-trehalose, melezitose, soluble starch, D-xylose, L-arabinose, D-arabinose, L-rhamnose (weak), D-ribose, ethanol, glycerol, ribitol (weak), D-mannitol, D-glucitol, arabinitol, D-gluconic acid, (slow), succinic acid, citric acid, arbutin, N-acetyl-D-glucosamine, D-glucosamine, D-glucono-𝛿-lactone, xylitol, 2-keto-D-gluconic acid, ethylamine (weak), L-lysine (slow and weak), nitrate and cadaverine. But lactose, melibiose, raffinose, inulin, methyl-α-D-glucoside, galactitol, erythritol, DL-lactate, methanol, D-glucuronic acid, myo-inositol, 5-keto-D-gluconic acid, hexadecane, and nitrite are not assimilated (Table 2 and Table S2). Growth in amino acid-free medium is positive, but no growth in vitamin-free medium. Growth on medium containing 50% glucose, 60% glucose and 10% sodium chloride/5% glucose is positive. Growth with 0.01 and 0.1% cycloheximide is positive. Grows at 25, 30, 35 and 37°C, but not at 40°C (Table 2 and Supplementary Table 2). Starch-like compounds and acid production are absent. Diazonium blue B colour and urease reactions are negative.
The type of N. odontotermitis f.a. sp. nov. is MTCC 13105T (=SMT1.3), was isolated from the gut of the termite O. horni in Kapare, Pune, Maharashtra (India). It is preserved in a metabolically inactive state at Microbial Type Culture Collection and Gene Bank (MTCC), Institute of Microbial Technology (IMTECH- Chandigarh, India), and also at the National Fungal Culture Collection of India (NFCCI), Pune, India (NFCCI 5012). The GenBank accession numbers of the ITS, LSU and SSU rDNA sequences are MZ234239, MZ234240 and OK384663. The Mycobank number is MB 841926.