In laser powder bed fusion (LPBF), defects such as pores or cracks can seriously affect the final part quality and lifetime. Keyhole porosity, being one type of porosity defects in LPBF, results from excessive energy density which may be due to changes in process parameters (laser power and scan speed) and/or result from the part’s geometry and/or hatching strategies. To study the possible occurrence of keyhole pores, experimental work as well as simulations were carried out for optimum and high volumetric energy density conditions in Ti-6Al-4V grade 23. By decreasing the scanning speed from 1000 mm/s to 500 mm/s for a fixed laser power of 170 W, keyhole porosities are formed and later observed by X-ray computed tomography. Melt pool images are recorded in real-time during the LPBF process by using a high speed coaxial Near-Infrared (NIR) camera monitoring system. The recorded images are then pre-processed using a set of image processing steps to generate binary images. From the binary images, geometrical features of the melt pool and features that characterize the spatter particles formation and ejection from the melt pool are calculated. The experimental data clearly show spatter patterns in case of keyhole porosity formation at low scan speed. A correlation between the number of pores and the amount of spatter is observed. Besides the experimental work, a previously developed, high fidelity finite volume numerical model was used to simulate the melt pool dynamics with similar process parameters as in the experiment. Simulation results illustrate and confirm the keyhole porosity formation by decreasing laser scan speed.