Bacterial cellulose (BC) has profound applications in different sectors of biotechnology due to its unique properties preferring it about plant cellulose. Although this polymer is extremely important in various applications, many problems still hinder the sustainable production in terms of increasing productivity and low-cost production. In order to overcome these problems, this study will focuses on the continuous production of cellulose using immobilized Glucanobacter xylinum cells onto Sugar Cane Bagasse (SCB) and Ca-alginate beads. Comparatively, adsorption of Glucanobacter xylinum cells to the cavum of stalk cells of SCB could be efficiently stable while, entrapment of cells onto Ca-alginate has drawback observed by the rapid disruption and instability of the beads in the Potato Peel Waste (PPW) culture medium. Our findings demonstrate that a combination between alternative low-cost medium with continuous production mode by immobilization onto inexpensive natural polymer can promote a sustainable bioprocess and reduction the production cost.