Aniline is a kind of refractory contaminant that is difficult to be degraded by microorganisms. Ozone is a green and efficient reagent to oxidize aniline, while the ozone oxidation efficiency is restricted by the low ozone mass transfer rate. Micro-nano bubble ozonation has been developed as a new method to significantly improve the ozone utilization rate, while the characteristics of ozone micro-nano bubble when compared with dissolved ozone is not clear. The paper carried out batch experiments to research the oxidation effect of aniline by ozone water (OW) and ozone micro-nano bubble water (OMNBW), and found that the degradation rate of aniline by OMNBW was 2.8~5.9% higher than that by OW. The increase of pH had a negative effect on the degradation of aniline by OW and OMNBW. SO42-, Cl-, HCO3- and Mg2+ could inhibit the degradation efficiency by 0.04%, 0.99%, 0.44% and 10.4% for OW, while the ratios were 1.1%, 6.4%, 4.1% and 1.5% for OMNBW. The addition of humic acid and fulvic acid could decrease the oxidation rate of aniline by 35% and 49% for OW, while the ratios were 41% and 62% for OMNBW. Through quenching experiment, it was found that the direct oxidation by ozone molecules and the indirect oxidation by superoxide radicals were main pathways for aniline oxidation by OW and OMNBW. This work provided a practical guide for the application of OMNBW in wastewater and groundwater treatment process.